摘要
本实验使用低浓度氚水(9.25×10^(?)-3.7×10~6Bq/mL)诱发中国仓鼠肺(CHL-1)细胞恶性转化,实验中作了不同时间的照射(24、48、72、96h),累积吸收剂量为0.055—0.88Gy。实验结果表明,氚水在此剂量范围引起该细胞的恶性转化率为3.28%—13.0%。实验中平行使用^(137)Csγ射线照射诱发该细胞恶性转化,剂量率为0.359Gy/d,照射时间与氚水组相同,累积吸收剂量为0.359—1.44Gy。γ射线在此剂量范围引起该细胞的恶性转化率为2.59%—13.4%。上述结果说明,两者的转化率与剂量相关。根据上述两种射线诱发 CHL-1细胞恶性转化率,求出氚水相对于γ射线的相对生物效应(RBE)为1.6。实验中同时作了形态学观察和细胞移植于动物的实验,实验结果都证明了转化了的细胞具有恶性细胞的形态和行为。
In this paper,the induction of neoplastic transformation in CHL-1 cells by low-dose-rate ex-posure to tritiated water was reported.CHL-1 cells were exposed to tritiated water(9.25×10~5—3.7×10~6Bq/mL)for 24—96 hours and the accumulated doses were estimated to be 0.055—0.88Gy,respec-tively.Neoplastic transformation was found in all expopsed cell groups.The morphological study andtransplantation test was carried out for demonstration malignancy of the transformed cells and the re-sults show that they are with the morphology and behaviour for malignant turnout cells.For CHL-1 cellsexposed to various doses of tritiated water,transformation rates wets found to be from 3.28% to 13.0% at doses of 0.055—0.88 Gy.In order to estimate RBE of tritium for malignant transformation inCHL-1 cells,the induction of malignant transformation in CHL-1 cells by exposure to ^(137)Cs gamma-rayswas carried out at dose rates of 0.359 Gy/24 hrs and transformation rates for irradiated CHL-1 cellswere found to bd from 2.59% to 13.4%.Based on these data,RBE of tritium for malignant transfor-mation in CHL-1 cells was estimated to be 1.6.
出处
《辐射防护》
CAS
CSCD
北大核心
1992年第3期196-200,共5页
Radiation Protection
关键词
氚水
仓鼠
肺细胞
细胞恶性转化
Tritiated Water
^(137)Cs Gamma-rays
CHL-l Cells
Cells Malignant Transformation
RBE
