摘要
本文提出了流动注射络合荧光法同时测定微量强力霉素和维生素B_2新方法。在中性条件下,维生素B_2在汞灯激发下于520hm发射荧光,而强力霉素与0.10mol/L MgSO_4溶液快速反应生成的荧光络合物也在520nm发射荧光,它们的荧光强度在维生素B_2和强力霉素浓度比为10∶1～1∶25范围内有加合性,由此同时测定了混合物中的维生素B_2和强力霉素。本法测定尿液中维生素B_2和强力霉素的回收率为95%～102％。
A new flow injection fluorimetric method for the simultaneous determination of doxycycline and vitamin B_2 is proposed. In neutral solution, vitamin B_2 emits fluorescence at 520nm when excited by Hg lamp, and doxycycline reacts with 0.10mol/L MgSO_4 to form fluorescence complex which emits fluorescence at 520nm. Their fluorescence intensities are of additive property in the doxycycline and vitamin B_2 concentrations ratios of 10:1～1:25, which has been used for the determinaton of doxycycline and vitamin B_2 in combina- tion. The recoveries of doxycycline and vitamin B_2 in urine are in the range of 95％～120％.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1992年第11期1261-1264,共4页
Chinese Journal of Analytical Chemistry
