摘要
目的 研究阿拓莫兰对临床体外循环 (CPB)患儿中性粒细胞核转录因子 (NF) -κB激活的干预作用。方法 随机抽取年龄 6～ 13岁先心病患儿 2 0例 ,配对分组为对照组 10例、干预组 10例 ,干预组CPB预充液中加入阿拓莫兰 30mg kg,分别于术前 2 4h、CPB开始 2 0min、复温前、CPB结束前、术后 4h、术后 2 4h抽取静脉血 ,分离中性粒细胞 ,提取核蛋白 ,以电泳迁移率实验测定NF -κB活性变化。结果 CPB开始 2 0min后 ,中性粒细胞NF -κB已被激活 ,在复温前达到峰值 ,术后 2 4h逐渐恢复至术前水平 ;干预组NF -κB活性下降 ,差异显著 ( P <0 0 5 )。结论 CPB过程可激活中性粒细胞NF -κB ,在中性粒细胞介导的CPB创伤、炎性反应中起重要作用 ;阿拓莫兰可抑制中性粒细胞NF -κB激活 ,减轻炎性反应。
Objective To study the intervention of redued glutathin in neutrophil nuclear factor-κB in the process of extracorporeal circulation. Methods 20 congenital heart disease children aged from 6 to 12 were selected randomly and were divided into control group (n=10) and intervention group (n=10). Patients of intervention group were added redued glutathin (30 mg/kg) to priming fluid, then phlebotomy was done at 24 h before operation, at 20 min after cardiopulmonary bypass (CPB), before rewarming, the end of CPB, 4 h after operation, 24 h after operation. After separating neutrophil and distilling nuclear protein, we mensurated the activation of nuclear factor-κB by EMSA. Results Neutrophil nuclear factor-κB has been activated at 20 min after CPB, and reached peak on the time of rewarming, and resumed at 24 h after operation in both groups but the intervention group was lower than the control group. Conclusion Neutrophil nuclear factor-κB was activated in the process of extracorpoeal circulation and played an important role in the inflammatory response happening in extracorporeal circulation, which could be relieved by redued glutathin.
出处
《中国急救医学》
CAS
CSCD
北大核心
2003年第2期82-83,共2页
Chinese Journal of Critical Care Medicine
