摘要
本文介绍酵母菌细胞脂肪酸的毛细管气相色谱分析,讨论了菌株培养物的皂化、脂肪酸甲酯的萃取、色谱柱的选择以及培养温度和培养时间对酵母菌细胞脂肪酸组分的影响。
A simple, rapid gas chromatographic method for the determination of cellular fatty acids from yeast species was described. Yeast strain was grown on SAB medium at 37℃ for 24h. The cellular fatty acid composition was ananlyzed by capillary gas chromatography (GC) after saponification of cells, methylation and extraction of methyl esters. Various conditions for sample preparation and GC analysis were compared. The effects of growth temperature and age on the cellular fatty acid compositions were also discussed.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1992年第10期1178-1180,共3页
Chinese Journal of Analytical Chemistry
关键词
细胞脂肪酸
酵母菌
气相色谱分析
Cellular fatty acids
Yeast
Capillary gas chromatography
