摘要
目的 :研究肝脏良恶性病变中细胞增殖活性和微血管密度表达强度与数量的差异性 ,进一步揭示肿瘤的生物学特征。 方法 :选取肝细胞癌 2 90例、肝硬化 12 8例与肝良性病变 (包括肝细胞腺瘤和肝细胞局灶性结节增生 ) 2 5例 ,应用组织芯片技术和免疫组化方法研究细胞核增殖抗原 (PCNA)、Ki- 6 7和微血管密度 (MVD)在肝脏良恶性病变中的表达强度与数量。结果 :PCNA、Ki- 6 7在肝细胞癌组阳性率分别为 90 .2 %和 4 3.1% ,在肝硬化组为 4 8.5 %和 3.9% ,组间差异有高度显著性(P<0 .0 1) ,而良性病变组无 1例阳性 ;MVD在肝细胞癌组为 2 2 .7± 15 .8,显著高于肝硬化组 (8.3± 7.6 ) ,差异有高度显著性(P<0 .0 1) ,而与良性病变组 (31.3± 2 2 .7)无差异 (P>0 .0 5 )。肝细胞癌病理 - 级 MVD(2 9.9± 18.6 )显著高于病理 级和 级 (2 2 .2± 18.2、2 2 .9± 19.0 ,P<0 .0 1)。结论 :细胞高增殖状态与多量的新生血管是恶性肿瘤的重要特征 ,PCNA、Ki- 6
Objective: To study the difference of the cell proliferation activity and microvessel density (MVD) between hepatic benign and malignant lesions for further demonstrating the biological features of tumor. Methods: There were 290 specimens of hepatocellular carcinoma (HCC), 128 specimens of cirrhosis tissues and 25 specimens of hepatic benign lesions were detected for PCNA, Ki 67 and MVD by immunohistochemistry on tissue microarray, respectively.Results: The expression level of PCNA and Ki 67 in HCC were 90.2% and 43.1%, which was obviously higher than that in cirrhosis (48.5% and 3.9%, P <0.01).There was no expression of PCNA or Ki 67 in benign lesions, MVD counting in HCC was 22.7±15.8, which was obviously higher than cirrhosis (8.3±7.6, P <0.01), but had no statistical difference with benign lesions(31.3±22.7, P > 0.05). MVD counting in HCC pathological grade Ⅰ Ⅱ(29.9±18.6) was higher than those in gradeⅢ (22.2± 18.2) and Ⅳ(22.9±19.0, P <0.01). Conclusion: The high cell proliferation and new angiogenesis are important features for malignant tumor. The expression of PCNA, Ki 67 and MVD may act as biological markers in differentiating malignant and benign hepatic lesions.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2003年第1期70-72,F003,共4页
Academic Journal of Second Military Medical University
基金
国家自然科学基金 (3 9970 72 8)
关键词
肝肿瘤
组织芯片
细胞增殖
微血管密度
liver neoplasms
tissue microarray
cell proliferation
microvessel density
