摘要
目的 探讨内皮素 (ET)在瘢痕成纤维细胞增殖与胶原合成中的作用及一氧化氮(NO)、粉防己碱 (Tet)的拮抗效应。方法 体外培养人增生性瘢痕成纤维细胞 ,以3H TdR掺入法测定细胞增殖 ;以3H 脯氨酸掺入量判断细胞胶原合成。结果 ET呈浓度依赖性促进瘢痕成纤维细胞增殖与胶原合成 ,随着ET浓度 (2 5～ 10 0ng ml)的增加 ,3H TdR掺入值较对照组分别增加了 1 8、4 0和 4 9倍 ;3H 脯氨酸掺入值较对照组分别增加了 1 1、3 1和 3 8倍 (P <0 0 1)。用NO供体亚硝基乙酰青霉胺 (S nitroso N acetylpenicillamine ,SNAP) 5 0 μg ml单独处理成纤维细胞 ,对3H TdR掺入值无明显影响 (与对照组相比 ,P >0 0 5 ) ,但对3H 脯氨酸掺入值有下调作用 ;SNAP可拮抗ET 1(2 5ng ml)刺激细胞增殖作用 (抑制率为 2 2 89% ,P <0 0 5 ) ;对ET 1的促胶原合成作用无明显影响 (P >0 0 5 )。Tet在不抑制细胞DNA合成的药物浓度 (3μg ml)即可明显减少瘢痕成纤维细胞3H 脯氨酸掺入值 ,并能显著降低由ET介导的瘢痕成纤维细胞3H TdR掺入值 (抑制率为 33 2 1% ,P <0 0 1)。结论 ET对体外培养的瘢痕成纤维细胞增殖与胶原合成具有显著促进作用 ,此效应可部分被SNAP、Tet所阻抗。
ObjectiveTo investigate the role of endothelin (ET) in the proliferation and collagen synthesis of human scar|derived fibroblasts and the modulation of its antagonists such as nitric oxide (NO), tetrandrine (Tet). Methods With the cultured fibroblasts from the scarring tissue, the cell proliferation was determined by [ 3H]|TdR incorporation, while the collagen synthesis was evaluated by [ 3H]|proline incorporation. Results The ET|1 was significantly increasing the proliferation and collagen synthesis of human scar|derived fibroblasts. The values of [ 3H]|TdR absorption in the 2.5 ng/ml, 25 ng/ml and 100 ng/ml of ET|1 groups were 1.8 times, 4 times and 4.9 times more than in the control group, respectively (P<0.01),while the values of the [ 3H]|proline incorporation were 1.1 times, 3.1 times and 3.8 times respectively (P<0.01). The fibroblasts, treated with 50 μg/ml of S|nitroso|N|acetyl penicillamine(SNAP), were no detectable effect on the basal level of DNA synthesis, but produced decreasing effect on the [ 3H]|TdR absorption (the rate of inhibition was 22.89%,P<0.05). It was found that the SNAP inhibited the [ 3H]|proline incorporation in cultured fibroblasts, but the rate of [ 3H]|proline incorporation induced by ET|1 was unaltered. The Tet with 3 μg/ml, in which does not inhibit the basal level of DNA synthesis, was significantly decreasing the collagen synthesis and decreasing the ET|mediated DNA synthesis (the rate of inhibition was 33.21%(P<0.01). ConclusionThese results indicate that the ET can obviously increase the proliferation and collagen synthesis of human scar|derived fibroblasts, but it can be partially antagonized by NO and Tet. ;
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2003年第1期51-53,共3页
Chinese Journal of Plastic Surgery
基金
江西省青年科学基金资助 ( 96 40 5 0 )
