摘要
为筛选丙型肝炎病毒 (HCV)核心蛋白(core)特异性噬菌体 12肽模拟表位 ,应用噬菌体表面展示技术 ,以抗 HCV核心蛋白抗原的单克隆抗体作为固相筛选分子 ,对人工化学合成的噬菌体随机 12肽库进行 5轮“吸附 洗脱 扩增”的筛选过程 ,随机挑取 5 0个克隆 ,经噬菌体酶联免疫吸附法 (ELISA)鉴定 ,并进行交叉反应实验以及竞争抑制性结合实验 ,最后对所选克隆进行DNA序列分析 ,以确定HCV核心抗原的模拟表位。经免疫学鉴定后 ,从随机筛选的 5 0个克隆中确定 10个阳性克隆 ,进行DNA序列测定 ,确定氨基酸序列XRQXXPXXXHXX为HCV核心的模拟表位。
To screen HCV core mimotopes,HCV core monoclonal antibody was used as immobilized molecule,and a 12 mer phage peptide library was biopanned and positive clones were selected by enzyme linked immunoadsorbent assay(ELISA), competitive inhibition assay, and DNA sequencing. 10 positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results,one epitope was confirmed as a mimotope of HCV core protein. The study might provide a new approach for HCV therapy and vaccine development.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2003年第1期34-36,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金资助课题 (编号C3990 0 1 30 )
