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反向遗传操作在新城疫病毒中的应用 被引量：1

Application of reverse genetic manipulation system used in NDV
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摘要 目前RNA病毒的反向遗传操作的发展非常迅速,已经在新城疫病毒得到应用。本文简述了新城疫病毒感染性分子克隆构建的基本机理,以及采用反向遗传操作技术利用新城疫病毒制备标记疫苗、研究病毒基因的结构与功能、制备表达外源基因的重组病毒等方面的研究进展。 The current techniqu e of genetic manipulat ion of RNA virus by reverse genetic system have developed quickly,it has been u sed in New Castle Disease Virus(NDV),this review lustrated the basic techniqu e to construct NDV from cloned cDNA,and development of this technique to study virus gene structure to functioin,construct marker vaccine,construct chimeric virus expressing exologus genes.This review summarized all the applicatioin an d development of reverse genetic system used in NDV.
作者 智海东 王云峰 童光志
机构地区 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室
出处 《畜禽业》 2003年第1期44-45,共2页 Livestock and Poultry Industry
关键词 反向遗传操作 新城疫病毒 应用 鸡 感染性分子克隆 reverse geneti c system,NDV,Marker Vaccine,recombinant chimeric virus
分类号 S852.65 [农业科学—基础兽医学]
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参考文献6

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同被引文献19

  • 1胡顺林,张艳梅,孙庆,刘玉良,吴艳涛,刘秀梵.鹅源新城疫病毒拯救体系的建立[J].微生物学通报,2007,34(3):426-429. 被引量：6
  • 2Neumann G, Whitt M A, Kawaoka Y. A decade after the generation of a negative-sense RNA virus from cloned cDNA - what have we learned[J]. J Gen Virol, 2002, 83(Pt 11): 2635-2662.
  • 3Peeters B P, Olav S de Leeuw, Koch G, et al. Rescue of Newcastle disease virus from cloned cDNA: evidence that cleavability of the fusion protein is a major determinant for virulence[J]. J Virol, 1999, 73(6):5001-5009.
  • 4Romer-Oberdorfer A, Mundt E, Mebatsion T, et al. Generation of recombinant lentogenic Newcastle disease virus from cDNA[J].J GenVirot, 1999, 80 (Pt 11): 2987-2995.
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  • 6Krishnamurthy S, Huang Z, Samal S K. Recovery of a virulent strain of Newcastle disease virus from cloned cDNA: expression of a foreign gene results in growth retardation and attenuation[J]. Virology, 2000, 278(1): 168-182.
  • 7Nakaya T, Cros J, Park M S, et al. Recombinant Newcastle disease virus as a vaccine vector[J]. J Virol, 2001, 75 : 11868- 11873.
  • 8de Leeuw O S, Koch G, Hartog L, et al. Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein[J]. J Gen Virol, 2005, 86: 1759-1769.
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畜禽业
2003年 第1期

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