摘要
本文以C57BL/6J鼠分2组进行实验。实验组45鼠,对照组20鼠,与同品系雄鼠交配后,在3日龄胚期,通过腹部手术对实验组雌鼠每侧子宫腔内注入未稀释的H-Y抗血清与豚鼠补体(二者以1:2混合)3μl。抗血清是由C57BL/6L雌鼠用同品系雄鼠的脾细胞以2.5×10~7个/只的剂量,每周免疫一次,共免疫7次后制备。对照组的处理操作与实验组相同,但每侧子宫腔内仅注入补体3μl,未注入抗体。实验组中有42只产仔247只(每窝平均6只),雌雄比为2.7(180/67)雌性占73％;对照组20只均产仔共163只(每窝平均8只),雌雄比为0.92(78/85),雌性仔鼠占48％。实验组雌性仔鼠百分率比对照组提高了25％。据X^2检验结果表明,实验组与对照组间的差异很显著(P<0.001)。
Fortvy-five C57BL/6J female miee were mated with the same strain mals . 3μ1 minure of undiluted H-Y antisera and guinsa pig compl numal(1:2) were injected into caeh merine horn of the female mice, while into cach utorine horn of control female mice only 3μ1 complement was iujceted. In conuqmunen. 247 offsprings were obtained from 42 of 45 experimebtal females micecach Liffer ha 6 offsprings on aware. he sax ralo was 2.7 (180 /67). and its perecnmage was 73%; 163 offsprings were obtained from 20 contrel femal moce the awrage of cach litter was 8 offsprings .the sae ratio was 0.92(78/85). and its perentage was 18%. Obviously. the perecntage of the fermer was 25% higher than that of the laner. the difference between them was wary signifieant (P<0.001).
基金
国家自然科学基金
国家"863"计划生物技术领域研究经费资助项目
