摘要
以微铺展—硝酸银染色技术制备三种鸡的SC标本,进行电镜观察。结果表明:三种鸡的SC组非常相似,即2n=82,ZZ/ZW型性别决定,雄性为ZZ。除1号SC和Z-SC为中着丝粒外,其余均为端着丝粒。Z-SC的相对长度有明显的细胞间差异;平均相对长度度介于第3和第4号SC之间。三者SC组型上的差异主要表现在相应SC长度上的不同。并对其亲缘关系及在鸟类进化中的可能地位进行了讨论。此外,在微铺展法制备的锦鸡精母细胞SC标本中还发现了巨大中心粒,这在高等动物尚属首次。
Micro-spread and silver-stained preparations of spermatocytes of C. am-herstiae, C. pictus and P. colchicus have been analyzed by electron micro-copy. SC karyotypes of three pheasants are very similar and are 2n=82, ZZ/ZW sex ditermined and male is ZZ. No.1 and Z-SC are metacentric, the others are telocentric. Relative length of Z-SCs has strikingly difference among spermatocytes, but the mean is always between No.3 and No.4 SC. The main diiterence of SC karyotypes of three pheasants is the length of corresponding SCs. The consanguinity and probable position of three pheasants in cvolution have been discussed. Large centrioles arc found for the first time in C. amherstiae and C. pictus spermatocytes and can be analyzed at the same time in SC samples prepared by microspreading technique.
关键词
中国雉鸡
白腹锦鸡
红腹锦鸡
SC
Chrysolo pints mnherslioc, Chrysolo phus pictus, Phusianus colchicius, Synaptoncmal complex (SC), Large centriole
