摘要
目的观察无特殊防护措施的职业性接触抗肿瘤药物所产生的遗传损伤。方法用胞质阻断微核试验(CBMN)和彗星试验(SCGE)检测某医院肿瘤科接触抗肿瘤药物的24名护士和非接触抗肿瘤药物的24名女医生(对照)外周血淋巴细胞的DNA和染色体损伤。结果暴露组微核细胞率为13.25‰±5.17‰,显著高于对照组3.79‰±2.19‰,P<0.01,暴露组彗星全长为(43.13±17.82)μm,也明显高于对照组(27.52±5.69)μm,P<0.01。结论肿瘤科护士外周血淋巴细胞的染色体和DNA已受到不同程度的损伤。
Objective To observe the genetic damages relate d to occupational exposure to antineoplastic agents without special protective measures in exposed subjects.Methods The damages of DNA and chromosomes of peripheral blood lym-phocytes were determined by comet assay and cytokinesis-block micronucleus test in exposure group consisting of24nurses oc-cupationally exposed to antineoplastic agents without special protective measures and control group consisting of24female doc-tors without exposure to antineoplastic agents in oncology department in a certain hospital.Results The exposure group showed significantly higher micronucleus rate(13.25‰±5.17‰)compared with that of control group(3.79‰±2.19‰),P<0.01.The mean full length of comet in exposure group[(43.13±17.82)μm]was also significantly higher than that of control group[(27.52±5.69)μm],P<0.01.Conclu sion The DNA and chromosomes of peripheral lymphocytes of nurses working in oncolo-gy department had been damaged in different degrees.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2003年第1期21-22,49,共3页
Journal of Environment and Health
基金
浙江省科技厅国际合作项目(012104)
关键词
护士
抗肿瘤药
DNA损伤
染色体畸变
职业性接触
Antineoplastic agents
DNA damage
Chromosome aberration
Occupational exposure
