摘要
Objective To study the effect of total body irra diation on the quantity and activity of P65,TNF-αand IL-1in irradiated rat wounds.Methods Tumor necrosis factor-α(TNFα)and interleukin-1(IL-1)in wound fluids were quantified by ELISAand their biological activities were measured by L929cell lysis and thymocyte proliferation,respectively.The expression of P65protein in wounded tissue was deter-mined by western blotting.Results The biological activities of TNF-αand IL-1in wound fluid decreased sig nificantly after irradiation,while their quantities in wound fluids did not decrease significantly.The exp ression of P65protein in wounded tis sue also did not decrease obviously a fter irradiation.Conclusion These results suggestted that total body irradiation may inhibit the bio logical activities of TNF-α,IL-1and nuclear factor-B,there may exist negative f actors to disturb the protein functions in wound environments.
Objective To study the effect of total body irra diation on the quantity and activity of P65,TNF-αand IL-1in irradiated rat wounds.Methods Tumor necrosis factor-α(TNFα)and interleukin-1(IL-1)in wound fluids were quantified by ELISAand their biological activities were measured by L929cell lysis and thymocyte proliferation,respectively.The expression of P65protein in wounded tissue was deter-mined by western blotting.Results The biological activities of TNF-αand IL-1in wound fluid decreased sig nificantly after irradiation,while their quantities in wound fluids did not decrease significantly.The exp ression of P65protein in wounded tis sue also did not decrease obviously a fter irradiation.Conclusion These results suggestted that total body irradiation may inhibit the bio logical activities of TNF-α,IL-1and nuclear factor-B,there may exist negative f actors to disturb the protein functions in wound environments.
出处
《中国临床康复》
CSCD
2002年第24期3780-3781,共2页
Chinese Journal of Clinical Rehabilitation
关键词
放射损伤
P65蛋白
TNFΑ
IL-1
TNF-α
IL-1
P65
wound healing
irradiat ion
delayed skin healing
