摘要
目的 研究羌菖合剂对脑供血不足大鼠外周红细胞的作用,探讨其改善血液流变性的原理。方法 手术结扎大白鼠右侧颈总动脉造成脑供血不足;术后3、7天仪器分析红细胞及其流变性参数。结果 (1)术后3d,对照组大鼠外周血红细胞数(RBC)、血红蛋白(HGB)和平均红细胞体积(MCV)与假术组无明显差异,平均细胞血红蛋白(MCH)、血球比积(HCT)和RBC聚集指数呈增高趋势,平均细胞血红蛋白浓度(MCHC)明显增大(P<0.05);羌菖合剂10g/kg治疗3d可显著防止上述改变(P<0.05～P<0.001)。(2)术后7d,对照组外周血红细胞及其血红蛋白量均比假术组无明显差异,但RBC刚性指数和聚集指数则呈进一步升高趋势;羌菖合剂治疗组MCHC、RBC刚性指数和聚集指数均显著低于对照组(P<0.05)。结论 羌菖合剂治疗能防止脑供血不足大鼠外周血红细胞改变;降低MCHC,降低RBC刚性指数和聚集指数,从而改善RBC变形能力和流变性。
Objective Effects of the decoction of Notopterygium incisium and Acorus gramineus(N-A-Dec)on peripheral RBC inexperimental right cerebral ischemic rats were studied; and the mechanism of N-A-Dec improving blood mobility was searched.Methods The right cerebral ischemia in rats were formed by ligating their right cervical artery; and on 3d and 7^(th) day after ligat-ing, the peripheral RBC and their mobility were analyzed with instruments. Results ①On the 3d day, the RBC-count(RBC),hemoglobin amount(HGB) and RBC mean volume(MCV) in the control group were about the same as that in the false-ligatedgroup; the mean RBC-hemoglobin (MVCH), blood cell ratio (HCT) and RBC gathering index tend to increase; and the mean RBChemoglobin concentration (MCHC) was greatly increased (P<0.05). These changes could be prevented significantly by treatingthe right CA ligated rate with N-A-Dec(10g/kg×3d) orally(P<0.05～P<0.001). ②On the 7^(th) day, the RBC, HGB, MCV,MCH and MCHC in control group had no remarkable differences to that in false-ligated group, but the RBC rigid index and gather-ing index tended to increase further. The MCHC, RBC rigid and gathering indexes were decreased remarkably in N-A-Dec treatedgroups. Conclusion The treatment of N-A-Dec to cerebral ischemic rats can prevent their changes in peripheral RBC, decreaseMCHC and RBC rigid and gathering indexes, and thus improve the transforming ability and mobility of RBC.
关键词
羌菖合剂
脑供血不足
大鼠
外周血
红细胞
Notopterygium incisium
Acorus gramineus
rat
cerebral ischemia
red blood cell
