摘要
目的 初步了解干细胞白血病 (SCL)基因在白血病和正常骨髓基质细胞中的表达情况 .方法 通过长期体外培养扩增骨髓基质细胞 ,运用RT -PCR -ELISA检测SCL基因的表达 .结果 SCL表达于绝大多数CML、AML、CLL患者骨髓基质细胞中 ,部分正常骨髓基质细胞也表达SCL基因 .
Objective To introductorily investigate the expression of SCL gene in leukemic and normal human bone marrow stromal cells.Methods Selectively augment the quantity of bone marrow stromal cells by long-term hematopoietic cells culture in vitro.The collected stromal cells were further detected the expression of SCL gene by RT-PCR-ELISA assay.Results The expression of SCL gene could be identified in the bone marrow stromal cells from most cases with CML,AML or CLL,and SCL gene could be detected in stromal cells from a part of normal individuals.Conclusions SCL may be involved in the regulation of hematopoiesis in leukemic and normal bone stromal cells.
出处
《现代临床医学生物工程学杂志》
CAS
2002年第6期406-408,共3页
Journal of Modern Clinical Medical Bioengineering
基金
国家攀登计划资助项目 (95 -专 - 1 0 )
