摘要
目的 检测燃煤性砷中毒患者外周血红细胞GPA的突变频率及其与病程的关系。方法 分离、固定4 0例燃煤性砷中毒患者外周血红细胞 ,与荧光素标记的单抗结合后 ,采用流式细胞术进行GPA变异分析 ,计算GPA变异频率。结果 4 0例燃煤性砷中毒患者外周血GPANN变异频率为 (2 1.2 3± 13.97)× 10 -6,NO变异频率为 (33.13± 2 5 .72 )× 10 -6,MM变异频率为 (110 .90± 6 3.5 8)× 10 -6,MO变异频率为 (2 0 .35± 2 1.2 6 )× 10 -6,GPA变异频率明显高于正常人群 (P <0 .0 1) ,但不同病程的燃煤性砷中毒患者之间的GPA变异频率未见明显差异。结论 砷中毒可导致人类外周血红细胞GPA的突变。
Objective Applying Glycophorin A (GPA) mutation assay in normal adults and patients with coal arsenic toxin , we may observe the frequency of variant erythrocytes at the glycophorin A locus and also the relation between the change of it and stage of disease. Methods Combination of immunolabeling and flow cytometry (FCM),18 normal adults and 40 patients were examined. Results GPA NN/NO/MM/MO variant frequencies(Vfs) of CAT patients were(21.23±13.97)×10 -6 ,(33.13±25.72)×10 -6 ,(110.90±63.58)×10 -6 ,(20.35±21.26)×10 -6 respectively ,significantly higher than that of normal subjects. No difference of variant frequendies was found in the patients with different courses(P<0.01). Conclusions The contact of arsenic may induce the mutation of erythrocytes at the glycophorin A locus ,suggesting that arsenic may be a kind of potential mutagen.[
出处
《合肥医学院学报》
2002年第6期498-500,共3页
Journal of Zunyi Medical University
基金
贵州省省长资金 (C 197)
贵州省教育厅基金 (C 174 )科研项目
