摘要
研究囊泡 囊泡素 1与清道夫受体A的相互作用及在动脉粥样硬化发生发展中的作用。将 16只正常小鼠随机分为正常对照组和高脂组 ,16只清道夫受体AⅠ转基因小鼠随机分为转基因组和转基因高脂组。正常对照组和转基因组用基础饲料喂养 ,高脂组和转基因高脂组用高脂饲料喂养。 2 4周后用免疫组织化学法检测小鼠主动脉血管上囊泡素 1的表达 ,计算机图像分析观察主动脉斑块面积及内膜 中膜厚度 ,电镜观察囊泡在主动脉内皮细胞膜上的表达。结果发现 ,高脂组、转基因组、转基因高脂组小鼠的内膜厚度和中膜厚度明显增加 ,是正常对照组的 2～ 4倍 ,3组均可见明显动脉粥样硬化斑块形成。正常小鼠主动脉血管表达高水平的囊泡及囊泡素 1,而转基因鼠囊泡及囊泡素 1表达明显减少。转基因鼠经高脂喂养后 ,上述表现更加明显。以上结果提示转基因清道夫受体AⅠ小鼠动脉粥样硬化的发生可能与其动脉内皮细胞上囊泡 囊泡素 1下调有关。
Aim To study the role of caveolae and caveolin 1 on atherosclerosis formation in scavenger receptor AⅠ (SR AⅠ) transgenic mice. Methods Wild type and SR AⅠ transgenic mice were randomly divided into control, transgenic, wild type plus high cholesterol diet, and transgenic plus high cholesterol diet groups, fed with normal or high cholesterol diet respectively. Animals were sacrificed after 24 weeks. The area of plaque was measured by electronic computer for image analysis. Immunohistochemical analysis and electron microscopy were used to investigate the expression of caveolae and caveolin 1. Results The atherosclerotic plaque were observed in wild type plus high cholesterol diet group, transgenic group, transgenic plus high cholesterol diet group. The thickness of intima and media were thinner in normal control group than in other three groups (P<0.05). Immunohistochemical analysis showed the caveolin 1 expression in above three groups were lower than that in control group. Furthermore, electron microscopy showed the expression of caveolae were also decreased. Conclusion Decreased expression of caveolae and caveolin 1 is associated with the atherosclerosis formation in SR AⅠ transgenic mice.
出处
《中国动脉硬化杂志》
CAS
CSCD
2002年第6期461-464,共4页
Chinese Journal of Arteriosclerosis
基金
国家 973项目 (G2 0 0 0 0 5 690 5)
国家自然科学基金(3 990 0 0 613 9970 847)资助
