摘要
目的 研究利多卡因对兔内毒素性急性肺损伤的预防作用。方法 雄性健康新西兰兔 2 4只 ,分 3组 :空白组 (S -S ,输注生理盐水 ) ,内毒素组 (L -S ,输注内毒素后输注生理盐水 ) ,预防组 (Ld2 -L ,输注内毒素前 10min输注利多卡因负荷量 2mg·kg- 1 ,然后 2mg·kg- 1 ·h- 1 ) ,控制呼吸 ,吸氧浓度 95 % ,输注内毒素后 6h杀兔。观察氧合指数 (PaO2 FiO2 )、肺动态顺应性 (Cdyn)、支气管肺泡灌洗液 (BALF)白蛋白含量、肺湿 干重比值、肺组织超氧化物歧化酶 (SOD)、丙二醛 (MDA)和髓过氧化物酶 (MPO)以及肺形态学变化。结果 内毒素组PaO2 FiO2 、Cdyn 和肺组织SOD活性明显下降 ;BALF白蛋白含量、肺湿 干重比值和肺组织MDA和MPO含量明显上升 ;肺泡结构严重毁坏 ,肺泡间隔增宽 ,大量炎症细胞浸润。利多卡因预防可明显减轻这些变化。
Objective To study the prophylactic effect of lidocaine on acute lung injury(ALI)induced by lipopolysaccharide (LPS) in rabbits.?Methods Twenty-four anesthetized male New Zealand rabbits were randomly assigned to receive one of the three treatments ( n =8 for each group): infusion of saline (group S-S), infusion of E.coli O111-B4 LPS (100 μg·kg -1 over a 60-min period)without lidocaine treatment (group L-S), and infusion of LPS with lidocaine treatment (group Ld2-L). A loading dose of lidocaine 2 mg·kg -1 was administered 10 min before infusion of LPS and thereafter at a rate of 2 mg·kg -1 ·h -1 . The lungs were ventilated with 95% oxygen. Hemodynamics, lung mechanics and PaO 2 were recorded. All animals were killed 6 h after the start of LPS infusion to study the activities of superoxide dismutase (SOD) and myeloperoxidase (MPO) and the content of methylenedioxyamphetamine (MDA) in the lung. The lung Wet/Dry weight ratio and albumin concentrations in bronchial alveolus lavage fluid (BALF) were analyzed as the indices of pulmonary edema. Finally, the lung tissue was sectioned for histological examination.?Results LPS caused decreases in PaO 2/FiO 2, C dyn and SOD in lung, and increases in lung Wet/Dry weight ratio, albumin in BALF, and MDA and MPO in lung. Pretreatment with lidocaine attenuated these changes. LPS caused extensive morphologic damages in the lung, which were attenuated by lidocaine pretreatment.?Conclusions Lidocaine has evident prophylactic effects on LPS-induced ALI in rabbits.
出处
《徐州医学院学报》
CAS
2003年第1期29-32,共4页
Acta Academiae Medicinae Xuzhou
关键词
急性肺损伤
内毒素
氧自由基
利多卡因
预防作用
acute lung injury
lipopolysaccharide
superoxide anions
lidocaine
prophylaxis
