摘要
目的 确定日本血吸虫可溶性尾蚴抗原作为血吸虫病早期诊断检测用抗原的应用价值。方法 从感染日本血吸虫的钉螺收集尾蚴 ,制备可溶性尾蚴抗原 (SCA) ,建立应用 SCA为抗原检测家兔血清抗体的 ELISA法 ,并以此法检测 2 8只低度感染日本血吸虫家兔的感染前和感染后不同时期的血清 ,并与常规 SEA-ELISA法比较。结果 用 SCA检测时 ,感染后 15 d有 2 /2 8只家兔血清开始出现抗 SCA抗体阳性 ,2 0 d已有 2 0只阳性 ,阳性率为 71.4% ,3 0 d 2 8只全部阳性 ,阳性率为10 0 % ,而抗 SEA抗体在感染后 2 0 d开始出现 ,3 4d有 2 1只阳性 ,阳性率为 75 .0 % ,3 7d阳性率为10 0 %。平均出现抗体的时间 SCA-ELISA和 SEA-ELISA分别为 (2 1.2 5± 4.0 7) d和 (3 1.5 0±5 .12 ) d,前者比后者早 10 d。
Objective To define early diagnosis value for schistosomiasis using the soluble cercariae antigen(SCA) detecting specific antibodies. Methods The soluble cercariae antigen(SCA) was prepared with cercariae collected from infected snails, and the SCA ELISA for detection of antibody was established. The antibodies to SCA in 28 sera of 28 infected rabbits before infection and at different times post infection were detected by SCA ELISA, and compared with SEA ELISA. Results The anti SCA antibodies could be detected earliest at day 15 post infection. Most of experimental rabbits(20/28) were positive of anti SCA antibody at day 20 post infection with SCA ELISA, at day 30 post infection all of rabbits were positive. But with SEA ELISA 21 of 28 rabbits were positive at day 34 post infection, until day 37 all rabbits were positive. The average time of antibody detected was 21 25±4 07 days with SCA ELISA, but 31 50±5 12 days with SEA ELISA. Conclusion The soluble cercariae antigen has a higher value for early diagnosis of schistosomiasis japonica.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
2002年第6期439-441,共3页
Chinese Journal of Schistosomiasis Control
基金
江苏省科技厅社会发展科技计划项目 [N o.BS2 0 0 10 6 1]
江苏省地方病协会血寄科研项目 ( X2 113)
