摘要
目的 :探讨人参皂苷Rg3的抗肿瘤血管形成作用。 方法 :用含不同浓度人参皂苷Rg3的培养液培养人肺癌SPC A 1细胞 ,收集培养上清并制备条件培养液 (CM ) ;将人脐静脉内皮细胞 (HUVEC)团培养在纤维蛋白凝胶中 ,在凝胶上面加入CM ,观察并记录内皮细胞形成的血管状结构数量。 结果 :来自肺癌细胞的CM能刺激内皮细胞呈管状结构生长 ,用人参皂苷Rg3处理肺癌细胞后的CM对管状结构生长的刺激作用减弱。 结论
Objectives: To investigate the effects of Ginsenoside Rg3 inhibiting tumor angiogenesis. Methods: Human lung cancer cells SPC A 1 were cultured in media containing Rg3,and conditioned media (CM) were prepared with the culture media from SPC A 1. Human umbilical vein endothelial cells (HUVEC) balls were cultured in collegen gel, and CM was put on collegen gel. The tubes from the HUVEC balls were noted. Results: CM from SPC A 1 cells culture media stimulated the tubes, and after SPC A 1 cells had been prereated with Rg3, the stimulation was attenuated. Conclusions: Ginsenoside Rg3 inhibits angiogenesis induced by tumor cell in vitro.
出处
《医学研究生学报》
CAS
2002年第6期493-495,共3页
Journal of Medical Postgraduates
