摘要
目的 探讨适合膜片钳技术的单离Hensen细胞的分离及对其钾通道的研究。方法 取豚鼠耳蜗 ,获得Corti器 ,采用酶消化和机械分离方法对Hensen细胞进行分离 ,并采用传统全细胞膜片钳技术 ,记录单离Hensen细胞的钾电流。结果 每只耳蜗可以获得活性良好的单离Hensen细胞 12± 3个。Hensen细胞的钾电流呈明显的外向整流性 ,只有迟电流 (IK) ,没有峰电流 (IA)。结论 酶消化结合机械分离的方法可获得适用于膜片钳技术研究的单离Hensen细胞 。
Objective To probe the method of isolating Hensen cells for patch-clamp technique and study potassium currents in isolated Hensen cells. Methods The organs of Corti of the guinea-pig cochlea were treated using enzyme, and then dissected. The whole cell patch-clamp technique was used to record potassium currents in Hensen cells. Results The number of living Hensen cells of one cochlea that was obtained was 12±3. The outward K+ currents was delayed rectified, TEA-sensitive potassium currents (I K), not rapidly inactive, 4-AP-sensitive potassium currents (IA).Conclusion Isolated Hensen cells for patch clamp are gained by enzyme digesting and mechanical dispersing. The properties of potassium currents in isolated Hensen cells are discussed.
出处
《听力学及言语疾病杂志》
CAS
CSCD
2003年第1期32-34,I002,共4页
Journal of Audiology and Speech Pathology
基金
国家自然科学基金资助项目 (编号 30 0 70 812 )
关键词
Hensen细胞
细胞分离
钾
膜片钳
Hensen cell Cell isolation Potassium Patch-clamp technique
