摘要
为了尝试用电子显微镜技术对基因进行定位,我们将pbGV基因组DNA经限制性内切酶EcoRI酶解后得到的E—V及E—I片段分别与感染晚期mRNA(主要成份为颗粒体蛋白mRNA及16K蛋白mRNA)杂交,用R—环法制备电镜样品。电镜观察表明,E—I和16K蛋白mRNA形成的R—环长度约0.1μ;E—V和颗粒体蛋白mRNA形成的R—环长度约0.3μ;电镜结果与生化试验结果相符。同时统计结果表明,颗粒体蛋白基因位于E—V片段的2/7处,16K蛋白基因位于E—I片段1/4处。
Gene location is one important and indispensable procedure for gene engineering. In order to try gene loca-tion with EM we made R-loop by the method of hybridezation of RNA to DNA. The material we used was pieris
Brassicase Granulosis Virus. Biollogical experiments proved the mRNA islocated and purified were the mRNA for granulin and for 16k.protein Under EM we observed there was R-loop formed between the mRNA for granulin and pbGV-DNA EcoRI-V fragment and other R-loop appeared between the mRNA for 16k protein and pbGV EcoRI-I fragment R-loops had the same length with their own mRNA.
出处
《电子显微学报》
CAS
CSCD
1992年第4期269-274,共6页
Journal of Chinese Electron Microscopy Society
关键词
电子显微镜
大菜粉蝶
病毒
定位
Microscopy
Granulin mRNA
Granulin gene
R-loop
Gene location
