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人纤溶酶原Kringle1基因的克隆与表达

Cloning and Expression of Human Plasminogen Kringle 1
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摘要 从人肝组织中抽提总RNA,通过RT-PCR方法扩增出人纤溶酶原(plasminogen)Kringle1片段,构建pPIC9K-K1载体,电击转化酵母,成功获得pPIC9K-K1/GS115工程菌.摇瓶发酵初步结果表明,工程菌能稳定表达可溶性的重组K1蛋白,分子量为15kD,发酵液中目的蛋白含量为84.17mg/L. Total RNA was extracted from human fresh liver. The gene encoding Kringle 1 of plasminogen was amplified by RTPCR. Then Kringle 1 gene was introduced into vector pPIC9K. Yeast cell GS115 was transfected by linear recombinant plasmid. Finally the yeast engineering cells named pPIC9KK1/GS115 was obtained stably expressing Kringle 1. Flask test showed that recombination protein K1 in culture supernatants reached up to 84.17 mg/L.
作者 陶亦敏 唐亮 张颉 谈立松 陈宇光
机构地区 上海大学生命科学学院 上海市第一肺科医院
出处 《上海大学学报（自然科学版）》 CAS CSCD 2002年第6期548-551,556,共5页 Journal of Shanghai University:Natural Science Edition
关键词 人纤溶酶原 KRINGLE 1基因 克隆 表达 抗肿瘤药物 plasminogen angiostatin Kringle 1 P.pastoris
分类号 R979.1 [医药卫生—药品] Q78 [生物学—分子生物学]
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上海大学学报（自然科学版）
2002年 第6期

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