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一种硫酸盐还原菌的亚硫酸盐还原酶基因(dsrA)的克隆和序列分析 被引量：2

Cloning and Sequencing of a dsrA Gene Encoding Dissimilatory Sulfite Reductase in a Sulfate Reducing Bacteria Consortium(SRB Ⅲ)
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摘要 硫酸盐还原菌是一类具有重要生态学意义的微生物.从一种具有还原六价金属铬能力的复合硫酸盐还原菌SRBⅢ中克隆了它的亚硫酸盐还原酶A亚基的完整基因.该序列带有亚硫酸盐还原酶 亚硝酸盐还原酶中保守的CX5CXNCX3C的序列.与已报道的同类基因相比,该基因与脱硫脱硫弧菌(Essex6株)的异化型亚硫酸盐还原菌最为接近,核苷酸序列的同一性为89%,氨基酸序列的同一性约为94%. Sulfate reducing bacteria is a group of microbes in the environment with important biological and ecological function. A DNA fragmentencoding the complete open reading frame of dissimilatory sulfite reductase alpha subunit from a sulfate reducing bacteria consortium (SRBⅢ) was cloned and sequenced. This open reading frame encodes a motif :CX5CXNCX3C,which is conserved in nitrite reductases and sulfite reductases. Compared with other know genes, it is most similar to the dissimilatory sulfite reductase gene from Desulfovibrio desulfricans(Essex 6), with a identity of 89% at nucleotide sequence and 94% at amino acid sequence deduced from DNA sequence.
作者 李亚萍 钟江
机构地区 复旦大学生命科学学院微生物和微生物工程系
出处 《复旦学报（自然科学版）》 CAS CSCD 北大核心 2002年第6期674-678,共5页 Journal of Fudan University：Natural Science
基金 国家自然科学基金资助项目(50130030) 上海宝钢集团公司资助项目
关键词 硫酸盐还原菌 异化型亚硫酸盐还原酶 序列分析 生物整治 sulfate reducing bacteria dissimilatory sulfite reductase DNA sequence bioremediation
分类号 Q933 [生物学—微生物学] Q939.1 [生物学—微生物学]
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参考文献10

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同被引文献37

  • 1冯颖,康勇,张忠国.硫酸盐生物还原体系的主要影响因素研究[J].水处理技术,2005,31(3):20-24. 被引量：13
  • 2陈虹,梅建凤,闵航.白蚁肠道微生物[J].微生物学杂志,2005,25(2):75-79. 被引量：32
  • 3张于光,李迪强,肖启明.分子生态学技术及其在环境微生物研究中的应用[J].微生物学杂志,2005,25(5):87-90. 被引量：6
  • 4王明义,梁小兵,郑娅萍,赵由之,魏中青.硫酸盐还原菌鉴定和检测方法的研究进展[J].微生物学杂志,2005,25(6):81-84. 被引量：13
  • 5魏利,马放,魏继承,李艳萍.大庆油田地面系统的硫酸盐还原菌的分离与鉴定[J].湖南科技大学学报（自然科学版）,2006,21(1):82-85. 被引量：8
  • 6Perez J R, Young L Y, Kerkhof L J. Molecular characterization of sulfate-reducing bacteria in anaerobic hydrocarbon degrading consortia and pure cultures using the dissimilatory sulfite reducible(darAB) genes [J]. FEMS Microbiol Ecol. ,2001, 35:145- 150.
  • 7Wagner M, Roger A J , Flax J L , et al. Phylogeny of dissimilatory sulfite reductases supports an early origin of sulfate respiration[J].J. Bacteriol., 1998,180:2975 - 2982.
  • 8Karkhoff-Sehweizer R R, Huber D P, Voordouw G. Conservation of genes for dissimilatory sulfite reductase from Desulfovibrio vulgaris and Archaeoglobus fulgidus allows their detection by PCR[J]. Appl.Environ. Microbiol., 1995,61:290 - 296.
  • 9Dahl C, Kredieh N M, Deutzmann R, et al. Dissimilatory sulfite reductase from Archaeoglobus fidgidus : physicochemical properties of the enzyme and cloning, sequencing and analysis of the reduetase gene[J]. J. Gen. Microbiol., 1993,139:1817 - 1828.
  • 10Joulian C, Ramsing N B, Ingvorsen K. Congruent phylogenies of most common small-subunit rRNA and dissimilatory sulfite reductase gene sequences retrieved from etuarine sediments[ J]. Appl. Environ.Microbiol. ,2001,67:3314 - 3318.

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复旦学报（自然科学版）
2002年 第6期

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