摘要
采用PCR技术对四川省近50年来年推广面积达66700hm2(100万亩)以上的40个小麦品种特异性位点(即STS-PCR标记)的遗传多样性进行了研究。13对STS-PCR引物和26种引物-酶组合中,92 3%的引物和88 5%引物-酶组合能揭示小麦品种间的多态性。在检测到的92条DNA片段中,86 96%具有多态性,平均每种引物-酶组合检测到3 08条(变幅为1～8条)。这些品种间遗传相似系数(GS)变幅为0 478～0 989,平均GS值为0 753。STS-PCR标记遗传距离聚类分析能将40个品种相互区分开,其结果与亲缘关系较一致。各年代品种间GS值变化趋势表明,从60年代后四川小麦品种的遗传多样性呈明显的下降趋势。
The genetic diversity among 40 Sichuan wheat (Tritcum aestivum L) cultivars, which has been popularized above 667×104?hm2 per year in Sichuan Province during recent 50 years, was investigated by using 26 primerenzyme combinations of 13 sequencetaggedsite (STSPCR) primer sets. 12 of 13 primer sets (923%) and 23 of 26 primerenzyme combinations (885%) reveal polymorphisms. A total of 92 restriction fragments has been detected in these cultivars, with 308 fragments per primerenzyme combination. The genetic similarity varies from 0478 to 0989 among 40 genotypes, with the mean of 0753. The results show that 40 genotypes can be distinguished by STSPCR markers. The variation of GS in 5 eras reveals that the genetic similarity has the tendency to increase since 1960.
出处
《四川农业大学学报》
CSCD
2002年第4期309-312,320,共5页
Journal of Sichuan Agricultural University
基金
国家教育部重点项目(00225)
四川省教育厅预研项目(2000-C02)
四川省科技厅农业生物技术专项基金资助。
