摘要
目的 :探讨金粉蕨素对氧化应激损伤人脐静脉内皮细胞的保护作用。方法 :以甲萘醌损伤人脐静脉内皮细胞 ,作为氧化损伤模型 ;采用MTT法 ,测定内皮细胞的生长抑制率 ;用硝酸还原酶法测定培养液中NO- 2 /NO- 3含量 ;以Westernblot方法检测细胞内皮型一氧化氮合酶 (eNOS)活性及小凹蛋白 (caveolin - 1 )表达。结果 :金粉蕨素呈剂量依赖性地降低甲萘醌对内皮细胞的生长抑制率 ,增加培养液中NO- 2 /NO- 3含量 ,保护eNOS活性、阻止caveolin- 1表达下降。结论 :金粉蕨素可能通过caveolin - 1
AIM: To investigate the protective effect of onychin on the endothelial cells injured by oxidative stress. METHODS: The injured model was established by endothelial cells treated with menadione. The growth inhibitory rate of endothelial cell was determined by MTT assay; NO - 2/NO - 3 concentration in the medium was determined by nitrate reductase assay; eNOS and caveolin-1 protein levels were determined by Western blot. RESULTS: Onychin significantly decreased the growth inhibitory rate of endothelial cells injured by menadione, increased NO - 2/NO - 3 concentration in the medium and eNOS activity and up-regulated caveolin-1 expression. CONCLUSION: Onychin possesses a protective effect against endothelial cell injury induced by menadione via caveolin-1/eNOS pathway.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2003年第1期10-13,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目 (No .39970 847)
湖南省卫生厅中医药科研课题 (No .2 0 0 0 0 0 6)
