摘要
以PCR方法扩增人bsp基因 ,与分泌型表达载体 pPICZαA重组 ,重组质粒经电击转化毕赤酵母GS115菌株 ,获得的GS115 /pPICZαA_hbsp表达菌株能高效分泌表达rhBSP .SDS_PAGE和Westernblot分析结果表明 ,产物的分子质量接近 6 6ku ,能发生特异性抗原
Human bsp gene was amplified by PCR and recombined with expressive vector pPICZαA.The recombinant vector pPICZαA_hbsp was transformed to Pichia pastoris GS115 by electroporation. The positive recombinant strains GS115/pPICZαA_hbsp screened can express and secrete rhBSP high effi_ ciently. SDS_PAGE and Western blot analyses showed that the expressed product was approximately 66 ku with specific antigenicity.
出处
《华南理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2003年第1期26-29,共4页
Journal of South China University of Technology(Natural Science Edition)
基金
广东省自然科学基金研究团队项目 (2 0 0 2 30 0 1)
