摘要
目的 探讨扩张型心肌病 ( DCM)易感的分子机制和确立一种较为实用的 MHC- 类基因的检测方法。方法 采用聚合酶链式反应和顺序特异性引物 ( PCR- SSP)基因分析方法 ,对 31例扩张型心肌病患者及 30例无血缘关系的健康人的人类主要组织相容性复合体 ( MHC- 类 ) DQ各等位基因及亚基因进行了检测分析 ,并将该方法与其它检测 MHC- 类基因的方法进行对比。结果 结果表明 ,MHC- DQB1* 0 30 1基因与 DCM呈正相关 ( P <0 .0 1) ,其它 MHC- DQB1*其它各等位基因未见异常。结论 MHC- DQB1* 0 30 1基因可能为北方汉族人 DCM的致病易感基因。尽管目前 MHC- 类基因分型方法已有多种 ,但所采用的方法 ( PCR- SSP)具有快速、简便、敏感、准确和可靠等优点。
Objective In order to study the correlativity between MHC-DQB1 gene polymorphism and Dilated Cardiomyopathy (DCM).Methods MHC-DQB1 gene polymorphism in 31 patients with DCM and 30 normal control subjects was analyzed by using the polymerease chain reaction sequence specific primer (PCR SSP)technique.Results The frequency of MHC-DQB1 0301 allele was significantly higher in patients than that in the controls,RR of DCM patients was,P<0.01.Conclusions These findings further verify that immunogenetics play a predominant pathogenetics role in a subset of DCM patients.Individuals carrying MHC-DQB1 0301 alleles may be susceptibility gene to DCM. This method is More specific, sensitive, simple,rapid and accurate,and could be applied to the routine clinical detective.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2003年第1期36-38,共3页
Chinese Jouranl of Endemiology
