摘要
目的 :探讨 10 2 3DNAzyme对HBV前C/C区mRNA的体外切割活性。方法 :用克隆及亚克隆技术将HepG2 2 15细胞中HBV前C/C区基因克隆入 pCDNA3 1(+)载体 ,转录表达该区mRNA。合成针对HBV前C/C区 2 0 31位点的 10 2 3DNAzyme,观察其对靶mRNA的切割活性。结果 :10 2 3DNAzyme对HBV前C/C区mRNA的切割效率与Mg2 + 离子浓度呈正相关。结论 :10 2 3DNAzyme能有效切割HBV前C/C区mRNA。
Objective To explore the use of synthetic 10 23DNAzyme targeted to PreC/C mRNA as potential therapy for hepatitis B,pCDNA 3 1(+) Pre C/C vector was established for transcriping target mRNA.Method Synthesize a 10 23DNAzyme corresponding to 2031 point of HBV PreC/C mRNA and observe its catalytic activity in vitro.Results Our results show that synthetic 10 23DNAzyme can efficiently cleave target mRNA in vitro,and its activity is dependent on the concentra tion of Mg 2+ ion.Conclusion The 10-23DNAzyme has the ability to specifically cleave HBV PreC/C mRNA with high efficiency under simulated physiological condition.
出处
《吉林医学》
CAS
2002年第6期326-327,共2页
Jilin Medical Journal
