摘要
目的 研究建立一种炎性胸膜腔积液的动物模型及探索巴曲酶对胸膜腔积液包裹的预防作用。方法 (1)选择 40只豚鼠分为实验组和对照组 ,分别于右胸腔注入 1%角叉菜胶 0 8～ 1 0ml和生理盐水 1 0ml,分批处死观察。 (2 )选择 60只豚鼠分为实验组及对照组 ,每只豚鼠右胸膜腔注入 1%角叉菜胶 0 8～ 1 0ml,实验组于注药后次日右胸膜腔注入 3巴曲酶单位 (3BU) ,对照组则注入生理盐水 1 0ml,分批处死对比观察。结果 角叉菜胶注射后 1天即出现胸膜腔积液 ,2～ 3天积液量最多 ,胸膜炎性改变亦达高峰 ,并逐渐出现胸膜纤维化粘连与包裹 ;而用巴曲酶后 ,胸液量明显减少 ,胸膜炎症反应减轻 ,胸液中纤维蛋白原 (Fbg)含量下降 ,组织纤溶酶原激活剂 (t PA)及D 二聚体 (D dimer)增加 ,未形成胸膜粘连及包裹。结论 使用角叉菜胶能够建立满意的豚鼠炎性胸膜腔积液模型 ;巴曲酶具有减轻炎症反应、减少胸膜腔渗液。
Objective To establish an animal model of inflammatory pleural effusion and to investigate the role of batroxobin in preventing encapsulation of pleural effusion Methods (1) Forty guinea pigs were divided into two groups: the animal model group received injection of 1% carrageenan (0 8～1 0 ml) in the right chest cavity; the control animals received injection of normal saline (1 ml) (2) Sixty guinea pigs were injected with 1% carrageenan (0 8～1 0 ml) in the right chest cavity,and then divided into a treatment group and a control group Batroxobin (3 BU) was injected into the right chest cavity of the animals in the treatment group,while normal saline was used in the control group The animals were killed at different time points Results In the animal model group, pleural effusion appeared in 24 hours and reached maximum amount within 2～3 days Neutrophils accumulated in the effusion and pleural inflammation intensified at the same time Fibrosis and pleural adhesion appeared by the seventh day,became prominent by the tenth day, and encapsulated pleurisy was evident by the fourteenth day With batroxobin treatment,pleural effusion and inflammation attenuated The fibrinogen (Fbg) level decreased,and the levels of tissue plasminogen activator (t PA) and D dimer increased in the effusion No pleural encapsulation was observed Conclusions Carrageenan was successfully used to establish an animal model of inflammatory pleural effusion in guinea pigs Batroxobin was effective in reducing inflammation and pleural effusion and in preventing pleural fibrosis and encapsulation
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2002年第12期736-738,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
