摘要
采用荧光定量法对转几丁质酶基因烟草T2植株的几丁质酶活性进行了测定,结果表明,转基因烟草株系的几丁质酶活性明显高于未转基因烟草,同时发现,pH对转基因烟草中的几丁质酶活性有一定的影响。对转基因烟草接种烟草炭疽病菌进行抗病性鉴定。
A fluorometric assay was used to confirm enzyme activity in chitinase gene transferred tobacco.Chitinase gene activity in transgene tobacco is higher than that in untransgene counterparts.Confocal laser scanning microscopy of foliar sections from a chitinase expressing Nicotiana tabacum Xanthinc embedded in (butylmethyl methacrylate) and stained with a polyclonal serum raised against the chitinase/fluorconjugated secondary(IgG) antibody detection system showed that chitinase was largely restricted to the vascular tissue,with a few accumulating in chloroplasts.Among the transgenic tobacco T2 plants inoculated with Colletotrichum nicotianae Averna,several lines displayed resistance to the fungus.
出处
《华北农学报》
CSCD
北大核心
2002年第4期1-4,共4页
Acta Agriculturae Boreali-Sinica
基金
国家烟草专卖局资助项目(981013)
留学回国人员科技择优资助
关键词
转几丁质酶基因
烟草
酶活性
抗病性
Tobacco
Chitinase gene
Enzyme activity
Fungal diseases resistance evaluation
