摘要
作者对32例肝病、血液病患者与5例丙氨酸转氨酶(ALT)异常供血员,应用二温度点多聚酶链反应(PCR)技术检测其血清的HCV RNA.结果发现,阳性血清在240 bp处有一条清晰的区带.37份血清的HCV DNA阳性率为48.6％.5例非甲非乙型输血后肝炎患者与3例急性非甲非乙型肝炎患者HCV RNA全部阳性,慢性肝炎与肝硬变患者中检出率也高(4/5).显示在我国各种类别的非甲非乙型肝炎中,丙型肝炎(丙肝)的比例很高.血液病患者与ALT异常的供血员中也有一定比例(分别为2/6与1/5)的丙肝病毒携带者,值得注意.结果提示PCR确是诊断丙肝特异而敏感的方法.
Sera from 32 patients with liver disease or hematonosis and 5 donors with elevation of alanine amino transferase (ALT) were tested for HCV DNA by two-temperature-point PCR. It was found that a clear band in the positive sample could be seen on gel. The positive rate of HCV RNA was 48.6% in 37 cases. All of 5 cases with non-A non-B post-transfusion hepatitis and 3 cases with acute non-A non-B hepatitis were HCV RNA positive. A high positive rate (4/5) of HCV RNA was also found among patients with chronic hepatitis or cirrhosis. These suggest that hepatitis C predominates in various types of non-A non-B hepatitis in China. It was worthy of note that the proportion of HCV carriers in the patients with hematonosis and the donors with elevation of ALT was 2/6 and 1/5, respectively. The findings suggest that the PCR is a specific and sensitive method for diagnosis of hepatitis C.
出处
《第四军医大学学报》
1992年第4期258-260,共3页
Journal of the Fourth Military Medical University
关键词
聚合酶链反应
RNA
丙肝病毒
polymerase chain reaction
hepatitis C virus
RNA
