摘要
为探讨HBxAg在慢性肝炎、肝硬变和原发性肝癌病变发生中的意义,作者以ABC和PAP免疫组化法研究了HBxAg以及HBsAg和HBcAg在肝及肝癌组织内的存在与分布。在110例慢性肝炎中,72(65.5％)例显示HBxAg阳性,66(60%)例HBsAg和35(31.8%)例HBcAg阳性。在108例肝硬变中,84(77.8%)例显示HBxAg阳性,73(67.6％)例HBsAg和18(16.7％)例HBcAg阳性。在110例原发性肝癌病例中,64(58.2%)例在肝癌组织内显示HBxAg阳性,17(15.5%)例HBsAg和12(10.9%)例HBcAg阳性;在80例癌周肝组织内,63(78.8%)例显示HBxAg阳性,47(58.8％)例HBsAg和21(26.3％)例HBcAg阳性。上述结果表明,在慢性肝炎、肝硬变和肝癌内HBxAg的检出率比HBsAg和HBcAg高,在肝癌组织内尤为明显。本研究证明了慢性肝炎、肝硬变及原发性肝癌和HBV慢性感染有密切关系。
The specimens of this study consisted of 110 cases of chronic hepatitis, 108 cases of liver cirrhosis and 110 cases of primary hepatic carcinoma (PHC). Formalin-fixed and paraffin-embedded sections were stained by ABC method for HBxAg, and for HBsAg and HBcAg with PAP method. Of the 110 cases of chronic hepatitis, 72 (65.5%) showed HBxAg positivity in the liver cells, 66 (60.0%) were of HBsAg and 35 (31.8%) were of HBcAg. Among the 108 cases of liver cirrhosis, 84 (77.8%) revealed HBxAg positivity in liver cells, 73 (67.6%) were demonstrated to be of HBsAg-positive cases and 18 (16.7%) were shown to be of HBcAg-positive cases. Among the 110 cases of PHC, 64 (58.2%) showed the HBxAg-positive reaction in cancerous tissues, and 17 (15.5%) for HBsAg and 12 (10.9%) for HBcAg in hepatoma cells were confirmed; sixty-three (78.8%) of the 80 cases of the cancerous surrounding hepatic tissues displayed the HBxAg positivity, 47 (58.8%) for HBsAg and 21 (26.3%) for HBcAg were also positive. In this study, the detection rate of HBxAg in chronic hepatitis, liver cirrhosis and PHC was higher than that of HBsAg and HBcAg. This appearance was very obvious in hepatoma tissues. The results of this study suggested that a relationship between chronic hepatitis, liver cirrhosis, PHC and chronic persistent infection of HBV is very close.
出处
《第四军医大学学报》
1992年第2期88-92,共5页
Journal of the Fourth Military Medical University
关键词
乙型肝炎
肝硬变
肝肿瘤
免疫学
hepatitis B
liver cirrhosis
hepatocellular carcinoma
hepatitis B antigens
hepatitis B surface antigens
hepatitis B core antigens
hepatitis B X antigens
immunohistochemistry
