摘要
本文选择、设计并合成了含天然HDAg上的部分片段的27肽,用于建立检测抗HD的ELISA方法。试验结果表明,包被的合成肽抗原与已知抗HD阳性冻存血清呈抗HD阳性反应;它能与天然HDAg竞争抗HD,具天然HDAg上相应片段的抗原活性;该合成肽抗原专一性强,与正常人血清,正常小鼠血清,单纯甲型、乙型、丙型肝炎抗体阳性血清无免疫交叉反应。用建立的ELISA法,检测1992年1～5月的部分献血员及住本科各型肝炎患者血清标本的抗HD。300例献血员中1例抗HD阳性(0.33％),其ALT增高2倍以上。非乙型肝炎41例,甲型肝炎52例,抗HD均阴性。各型乙型肝炎211例,抗HD阳性21例(9.95％)。其中,携带者2/82例(2.5％);急黄肝6/43例(13.95％);慢活肝6/60例(10.00％);重肝4/18例(22.20％);肝炎肝硬化3/8例(37.50％),结果与本科已往报告基本一致。
A 27-peptide,a fragment of hepatitis delta antigen(HDAg),was synthesized and used to develop an ELISA method for ihe detection of anti-HD.It was found that positive anti-HD reaction occurred between the coated 27-peptide and a stored sample of serum which was known anti-HD positive.Absorption test revealed that the synthetic peptide competed with natural HDAg for anti-HD,suggesting that the peptide possessed the antigenicity similar to that of natural HDAg.The antigenicity of the synthetic peptide was quite specific wihtout cross reaction with normal human and mouse sera and with anti-HA.anti-HB and anti-HC sera.Among 300 blood donors,there was only 1 case(0.33%)anti-HD positive with an ALT level 2 times higher than normal.In 41 cases of non-B hepatitis and 52 cases of HAV hepatitis,none was anti-HD positive.In 211 cases of various types of HBV hepatitis,21 were(9.95%)anti-HD positive,among whom 2/82(2.5%)werehealthy HBV carriers,6/43(13.95%)were patients with a-cute icteric hepatitis,6/60(10.00%)were patients of chronic active hepatitis,4/18(22.20%)were patients of severe hepatitis,and 3/8(37.50%)were those with liver cirrhosis.These results were consistent with those in our previous reports.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1992年第5期415-418,共4页
Journal of Third Military Medical University
