摘要
建立原药材制首乌中主要有效成分 2 ,3,5 ,4’ -四羟基二苯乙烯 - 2 -O - β -D -葡萄糖苷的含量测定方法 ,以C1 8为固定相 ( 1 0 μm ,1 3 9mm× 30 0mm) ,乙腈—水 ( 2 5∶75 )为流动相 ,32 0nm检测 ;流速∶0 8mL·min- 1 ;柱温∶室温 ;线性关系考察 ,回归方程为 :Y(X) =5 1 5X +35 1 8 66(r=9998,α =0 0 1 ) ,加样回收率平均值为 98 64% ,RSD =0 36% (n =5 ) .本法快捷、简便、准确 ,为制首乌的质量评价提供了有效的方法 .
The main effective component 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside in polygonum mutiflorum thunb was determined by HPLC under the conditions of C 18 Column (10 μm,13.9 mm×300 mm) with mobile phase of acetonitrile-water (1∶3),at 320 nm,flow rate 0.8 mL·min -1 ,room temperature as columm temperature.The calibration equation was Y(X)=515X+3518.66,with a correlation coefficient of 0.9998,a= 0.01. The average recovery rate was (98.64±0.36)%.The method is simple and accurate,and can be used for the quality control of raw medicinal material.
关键词
高效液相色谱法
测定
制首乌
二苯乙烯甙
中药
polygonum mutiflorum thunb
2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside
HPLC
