摘要
目的 探讨消旋聚乳酸 /羟基磷灰石 /脱钙骨基质 (PDL L A/ HA/ DBM)的制备方法及其体外降解特性。方法 按重量比 PDL L A∶ HA∶ DBM=1.5～ 2∶ 1～ 1.5∶ 1,应用乳液共混法 ,制备 PDL L A/ HA/ DBM生物复合材料 ,通过体外降解实验 ,观察其在磷酸盐缓冲液 (PBS,p H 7.4 )中重量、生物力学及 p H值的动态变化。结果 采用乳液共混法 ,不需加温 ,可将 PDL L A、HA、DBM一次性复合 ,制成生物复合材料 PDL L A/ HA/ DBM。孔隙率为 71.3% ;扫描电镜显示复合材料孔径为 10 0～ 4 0 0 μm;降解过程中 ,PDL L A溶液的 p H值在 2周内轻度下降 ,而后明显下降 ,4周时p H值为 4 .0 ;PDL L A/ HA/ DBM溶液的 p H值在 12周的降解过程中十分稳定 ,12周时 p H值为 6 .4。 PDL L A重量在 0～ 4周时变化较小 ,4周后下降加快 ,12周时为初始重量的 5 0 % ;PDL L A/ HA/ DBM重量在 0～ 5周时变化较小 ,5周后下降加快 ,12周时为初始重量的 6 0 %。PDL L A的初始抗压强度为 1.33MPa,PDL L A/ HA/ DBM的初始抗压强度为1.71MPa,两者有统计学意义 (P<0 .0 5 ) ;PDL L A强度在 0～ 3周下降较小 ,3周后下降较快 ,12周仅 0 .11MPa;PDL L A/ HA/ DBM强度在 0～ 4周下降较小 ,4周后下降较快 ,12周时为 0 .2 1MPa。结论 乳液共混法是?
Objective To explore a new method of preparing the composite of DL polylactic acid (PDLLA), hydroxyapatite(HA), decalcium bone matrix (DBM), and to observe the degradation characteristics of PDLLA/HA/DBM in vitro. Methods An emulsion blend method was developed to prepare the composite of PDLLA/HA/DBM based on the weight rate of PDLLA∶HA∶DBM=1.5 2∶1 1.5∶1. The characteristics of the particles was observed by scanning electron microscope. In vitro, PDLLA/HA/DBM and PDLLA were put into PBS(pH7.4) respectively; the pH value, weight and biomechanics of them were determined during the degradation. Results Without heating, the emulsion blend method could be developed to prepare PDLLA/HA/DBM. Scanning electron microscope showed that the gap diameter in the compound material was 100 to 400 μm, and the porosity was 71.3%; During degradation, the pH value of PDLLA decreased little within 2 weeks, then decreased obvioushy and decreased to 4.0 at the end of the 4th week; while the pH value of PDLLA/HA/DBM kept quite steady and was 6.4 at the end of the 12th week. The weight of PDLLA decreased little within 4 weeks, then decreased obviously and remained 50% of its prime weight at the end of the 12th week; while the weight of PDLLA/HA/DBM decreased little within 5 weeks, then decreased obviously and remained 60% of the prime at the end of the 12th week. The prime biomechanical strength was 1.33 MPa in PDLLA and 1.71 MPa in PDLLA/HA/DBM. There was significant difference between them ( P< 0.05). The strength of PDLLA decreased little within 3 weeks, then decrease obviously and was 0.11 MPa at the end of the 12th week; the strength of PDLLA/HA/DBM decreased little within 4 weeks, then decrease obviously and was 0.21MPa at the end of the 12th week. Conclusion The emulsion blend method is a new method to prepare bone repair materials. As a new bone repair material, PDLLA/HA/DBM is suitable for bone tissue engineering for its good characteristics of porosity and degeneration.
出处
《中国修复重建外科杂志》
CAS
CSCD
2003年第1期61-64,共4页
Chinese Journal of Reparative and Reconstructive Surgery
