摘要
目的 探讨成骨细胞复合 Pluronic F- 12 7表面修饰生物衍生骨的三维培养 ,对成骨细胞活力及功能表达的影响。 方法 将新鲜猪肋骨加工制成生物衍生骨 ,应用 Pluronic F- 12 7对生物衍生骨进行表面修饰 ,然后体外复合第 3代成骨细胞三维培养。实验为 A、B和 C组。A组为单纯生物衍生骨复合成骨细胞组 ,B组为 Pluronic F- 12 7表面修饰生物衍生骨复合成骨细胞组 ,C组为单纯成骨细胞组。应用倒置相差显微镜和扫描电镜对各组成骨细胞生长及黏附进行观察 ,并对其细胞活力和碱性磷酸酶 (AL P)活性进行检测。 结果 B组成骨细胞在支架材料上黏附、伸展及生长良好 ,成骨细胞活力和 AL P活性表达与 A组成骨细胞比较均无统计学意义 (P>0 .0 5 ) ;A、B组与 C组比较 ,成骨细胞活力和 AL P活性均明显降低 ,有统计学意义 (P<0 .0 1)。 结论 生物衍生骨经 Pluronic F- 12 7表面修饰后具有良好的细胞相容性 ,可作为负载生物活性分子的载体修饰生物衍生骨。
Objective To investigate the effects of three dimentional culture in bioderived material modified by Pluronic F 127 on the growth and function of rabbit periosteal osteoblast in vitro. Methods Bio derived materials were from fresh pig ribs and were modified by Pluronic F 127. Then rabbit periosteal osteoblasts were cultured in bio derived materials(group A), in the modified bio derived materials(group B) and on the plastic surfaces as a control (group C) , respectively. During a 7 day period, the status of growth, cell viability and alkaline phosphatase(ALP) activity were measured. Results Osteoblasts attached, elongated and grew well on the modified bio derived materials. There were no significant difference in osteogenesis and ALP activity between group A and group B( P >0.05). The osteogenesis and ALP activity in groups A, B were less than those in group C ( P< 0.01). Conclusion Pluronic F 127 can be used for a carrier for bioactive factors to modify bio derived material.
出处
《中国修复重建外科杂志》
CAS
CSCD
2003年第1期52-55,共4页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家高技术研究发展规划资助项目 (863 )(2 0 0 1 AA2 1 60 1 1 )
国家自然科学基金重点资助项目 (39830 1 0 0 )
