摘要
目的 :建立小鼠胚胎干细胞饲养层培养体系。方法 :用 5种不同鼠胚成纤维细胞为饲养层 ,进行小鼠胚胎干细胞的分离培养 ,观察 5种饲养层培养体系对小鼠胚泡发育、内细胞团增殖及胚胎干细胞分离培养的作用。结果 :原代或冻存复苏后的原代培养鼠胚成纤维细胞用于制备饲养层 ,有利于胚泡的贴壁、孵化 ,内细胞团增殖形成巢式生长集落 ,离散后培养 ,可以观察到胚胎干细胞集落的出现 ,并可在短期内维持胚胎干细胞的正常形态 ,不发生分化 ,与其他三组有明显差异。结论
Objective:To establish feeder layer for mouse embryonic stem cell. Methods:Isolated and culturied mouse embryonic stem cell using 5 different kinds of mouse embryonic fibroblasts as feeder layers,and the effects of different feeder layers on the development of mouse blastocyst,the proliferation of inner cell mass and the culture of mouse embryonic stem cell were observed. Results:The feeder layer of the primary or the thawed primary mouse embryonic fibroblast improved the hatching of blastocyst and the proliferation of inner cell mass into nest like clone. After being disaggregated,ES clones appeared. Moreover it maintained the stem cell like morphology in a short period which was evidently different with other 3 groups. Conclusion:The feeder layer of the primary or the thawed primary mouse embryonic fibroblast is a effective culture system of the isolation and culture of mouse embryonic stem cell.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2002年第5期437-441,共5页
Chinese Journal of Anatomy
