摘要
目的:探讨舌癌化疗的作用机制。方法:60例患者的术后石蜡标本分为3组,其中舌癌化疗组10例,舌癌组30例,舌乳头状瘤组20例。采用原位末端标记法和免疫组织化学染色,并检测舌癌化疗后的细胞增殖、细胞凋亡及Bcl-2表达。结果:舌癌组细胞增殖指数(PI)显著高于乳头状瘤组(P<0.05),而细胞凋亡指数(AI)低于乳头状瘤组(P<0.05);舌癌化疗组与舌癌组比较,AI显著升高(P<0.05),PI显著降低(P<0.05);舌癌化疗组与舌癌组比较,Bcl-2阳性细胞指数明显降低(P<0.05);舌癌化疗组中PI与AI呈负相关(r=-0.739),Bcl-2阳性细胞指数与AI呈负相关(r=-0.787)。结论:化疗药物抑制肿瘤生长的机制可能与抑制肿瘤细胞增殖和诱导肿瘤细胞凋亡有关,这种作用可能是通过下调Bcl-2表达水平实现的,提示舌癌术前化疗对肿瘤的预后有重要意义。
Objective:The purpose of this study was to investigate effects of chemotherapy on tongue squamous cell carcinoma by detecting the apoptosis, proliferation and expression of Bcl-2.Methods: A total of 60 samples were studied, including tongue squamous cell carcinomas with chemotherapy (10 cases), tongue squamous cell carcinomas (30 cases), tongue squamous cell papillomas (20 cases) . The samples were investigated using immunohistochemical stain and in situ detection technique. Results: The apoptosis index (AI) was significantly lower in the group of squamous cell carcinomas compared with the squamous cell papillomas, but the proliferation index (PI) in the former group was significantly higher than the latter( P < 0.05). Compared with the group of squamous cell carcinomas, the apoptosis index increased, but proliferation index decreased in the group of chemotherapy ( P < 0.05). Bcl-2 positive cell index (BI) in the group of squamous carcinomas significantly decreased after chemotherapy (P < 0.05). hi the group of squamous cell carcinomas with chemotherapy, AI was negatively correlated with PI (r= -0.739) and, BI was negatively related with AI (r= -0.787) . Conclusion: The mechanism of chemotherapy is associated with inhibition of proliferation and induction of apoptosis, which may be determined by decreasing the expression of Bcl-2. It indicates that the pre-opera-tive chemotherapy is important for clinical prognosis of patients with tongue squamous carcinomas.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2002年第6期414-416,共3页
West China Journal of Stomatology
