摘要
目的 研究尿激酶型纤溶酶原激活剂 (uPA)和其特异性受体 (uPAR)在肝细胞癌 (HCC)的表达及其临床和病理意义。 方法 应用免疫组织化学和酶联免疫吸附法 (ELISA)检测uPA和uPAR在HCC和非癌组织中的表达 ,并与临床及病理指标相联系。 结果 HCC中uPA和uPAR的阳性表达率分别为 75 0 % (12 / 16 )和 6 8.8% (1/ 16 )。在进展性和侵袭性肿瘤与非进展性和非侵袭性肿瘤间uPA和uPAR阳性率无显著性差异 (P >0 .0 5 )。阳性染色主要位于癌细胞和基质细胞胞浆且主要位于癌侵犯的边缘。非癌组织仅见少量阳性染色细胞 ,对照组无特异性阳性染色。在ELISA中 ,癌与非癌组织uPA抗原水平分别为 (4 2 3± 0 5 7)ng/mg蛋白和 (1 2 6± 0 14)ng/mg蛋白 ,uPAR抗原水平分别为 (4 2 5± 0 2 1)ng/mg蛋白和 (3 15± 0 2 3)ng/mg蛋白 ,有显著性差异 (t=18.96 3,P =0 .0 0 0 ;t=13.6 93 ,P =0 .0 0 0 )。且进展性和侵袭性肿瘤中其抗原水平显著高于非进展性和非侵袭性肿瘤 (P <0 0 5 )。 结论 uPA和uPAR在肝细胞癌表达较高并与其进展和侵袭密切关联。
Objective To investigate expression of urokinase-type plasminogen activator (uPA) and its receptor (uPAR) in hepatocellular carcinoma(HCC) and its clinical and pathological significance. Methods The expression of uPA in cancer and non-cancer samples of 16 cases of hepatocellular carcinoma was detected by immunohistochemistry and ELISA. Clinical and pathological features were associated with experimental data. Results The rates of positive expression of uPA and uPAR in cancer tissues were 75 0%(12/16) and 68.8%(11/16) respectively. The rates of positive uPA and uPAR between progressive and aggressive tumors and non-progressive and non- aggressive tumors didn't show significant difference ( P >0.05). Positive stains were located in cytoplasm of cancer and stromal cells, especially in invasive margin of cancer. Slight positive stains were observed in non-cancer tissues. But in the control samples, there wasn't specific stain. In ELISA, the uPA antigen levels in cancer and non- cancer tissues were (4.23±0.57)ng/mg protein and (1.26±0.14)ng/mg protein; the uPAR antigen levels in caner and non-cancer tissues were(4.25±0.21)ng/mg protein and (3.15±0.23)ng/mg protein respectively. Significant difference existed in both uPA and uPAR antigen levels in these two kinds of tissue ( t=18.963,P=0.000;t=13 693,P=0 000 ). Furthermore, these antigen levels in progressive and aggressive tumors significantly higher than those in non-progressive and non-aggressive tumors ( P <0.05) Conclusions uPA and uPAR have high expression in HCC tissues and close relations with the progression and aggression of HCC.
出处
《中国微创外科杂志》
CSCD
2002年第6期422-423,共2页
Chinese Journal of Minimally Invasive Surgery
