摘要
采用饲毒烟蚜(MyzuspersicaeSulzer)和汁液摩擦传毒的方法,对22种烟蚜田间常见的中间寄主进行接种,接种后7d,用无毒烟蚜接种中间寄主饲养2d后回接到健康烟株。观察烟株是否发病。经多次重复试验,最终确定小酸浆(PhysalisangulataL)和龙葵(DaturastramoniumL)为烟草丛顶病的中间寄主。采用汁液传毒中间寄主并用蚜虫回接烟苗未出现发病症状。
22 plant species of field common intermediate host for tobacco aphids were inoculated by means of viruseinfected tobacco aphids feeding and succus friction spreading.After one weak of inoculation, virusfree tobacco aphids were inoculated with the intermediate host plants mentioned above for 2 days,then reinoculated the healthy tobacco plants and the disease incidence of the tobacco plants was investigated.Through many repeated experiments physalis angulata L. and Datura stramonium L were finally identified as the intermediate hosts for tobacco witches' broom disease.The tobacco seedlings were not infected by succus friction spreading virus to intermediate hosts and aphids reinoculation.
出处
《云南农业大学学报》
CAS
CSCD
2003年第1期39-41,共3页
Journal of Yunnan Agricultural University
关键词
烟草
丛顶病
中间寄主
小酸浆
龙葵
tobacco
witches' broom disease
intermediate host
identification
