摘要
目的 分析广东省 1979～ 1999年不同地区登革Ⅰ型病毒地方分离株和国外毒株之间的遗传关系 ,并探讨其可能来源。方法 提取病毒RNA ,用一步法逆转录聚合酶链反应 (RT PCR)扩增E/NS1连接区基因片断 ,克隆于pBluescriptⅡSK质粒上进行序列测定 ,并将测序结果与Genbank中其他登革Ⅰ型病毒E/NS1基因片段的 2 4 0bp核苷酸进行比较 ,用计算机软件分析结果。结果 广东省分离到的 14株登革Ⅰ型毒株中 ,基本可分为两个基因亚型 ,它们与印度尼西亚毒株的核苷酸最大同源性为 99 2 % ,与菲律宾毒株的核苷酸最大同源性为 10 0 % ,与泰国毒株的核苷酸最大同源性为 98 8%。结论 广东省的登革Ⅰ型病毒可能来源于菲律宾、印度尼西亚和泰国等东南亚和西太平洋国家。
Objective To analyse the genetic relationship of local strains of dengue type 1 viruses isolated in different years and regions from Guangdong Province,and to explore the genetic links with strains of adjacent countries by comparing with the sequences of relevant strains in Genbank.Methods The viral RNAs were extracted and used for one step reverse transcriptase polymerase chain reaction(RT PCR)to amplify the partial nucleotide fragments in E/NS1 gene junction which were then cloned into the plasmid pBluescript Ⅱ SK for sequencing,the results were analysed by DNASTAR software. Results The phylogenetic tree of the sequenced 14 stranis of dengue type 1 viruses branches into two genotypic groups.The nucleotide sequences showed a maximal homologies of 99 2% with Indonesia strains,100% with Philippines strains and 98 8% with Thailand strains.Conclusion The dengue type 1 viruses of Guangdong Province are closely related to the Philippines,Indonesia and Thailand strains,which may indicate the possibility of importation from those countries.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2002年第4期382-384,共3页
Chinese Journal of Experimental and Clinical Virology
基金
广东省医学科学技术研究基金资助 (A19980 81)
