摘要
为了研究野生型 p53基因转染的HL 60细胞端粒酶活性和人端粒酶逆转录酶 (hTERT)基因表达的变化 ,采用脂质体法将野生型 p53基因转染HL 60细胞 ,用TUNEL检测细胞凋亡 ,用端粒重复扩增法 (TRAP) 酶联免疫吸附试验 (ELISA)检测端粒酶活性变化 ,以及用RT PCR方法检测hTERTmRNA表达的变化。结果显示 :转染野生型p53基因的HL 60细胞在 32 .5℃培养 2 4小时和 72小时后 ,凋亡率分别为 8.3 %和 2 1 .0 % ,hTERTmRNA和端粒酶活性分别下降至对照组的 68.4%和 55 .8%及 2 7.3 %和 8.9%。结论 :p53基因能下调HL 60细胞hTERTmRNA和端粒酶活性 。
To investigate the change of telomerase activity and human telomerase reverse transcriptase(hTERT) gene expression in HL 60 cells transfected with wild type p53 gene, wild type p53 gene was introduced into HL 60 cells by Lipofectin transfection. Apoptosis was analyzed by TUNEL assay. Telomerase activity and the level of hTERT mRNA were detected by telomeric repeat amplification protocol(TRAP) ELISA and RT PCR, respectively. The results showed that the apoptotic rate of HL 60 pN53cG cells was 8.3% and 21.0% respectively after cultured at 32.5℃ for 24h and 72h. The level of hTERT mRNA was decreased to 68.4% and 55.8% and telomerase activity to 27.3% and 8.9% of control value in HL 60 pN53cG cells at the same points. In conclusions, hTERT mRNA and telomerase activity were down regulated in HL 60 cells transfected with p53 gene. This may be one of mechanisms of apoptosis induced by wild type p53 gene.
出处
《中国实验血液学杂志》
CAS
CSCD
2002年第6期527-530,共4页
Journal of Experimental Hematology
