摘要
目的:反相高效液相色谱法测定心脑康胶囊中芍药苷的含量。方法:以50％乙醇超声提取,定容,过滤后直接进样。采用ODS-C_(18)色谱柱(4.6mm×150mm,粒径5μm);流动相采用乙腈-0.05％磷酸溶液(13:87);流速0.8mL·min^(-1);柱温20℃;检测波长:230 nm。结果:平均加样回收率为99.9％,RSD为0.90％(n=5)。结论:本方法灵敏,简便,重现性好,可有效地控制制剂质量。
OBJECTIVE An assay method was established for the determination of paeoniflorin in Xinnaokang capsules by RP-HPLC. METHODS The chromagraphic conditions were listed as follow; column ODS-C18(5μm,4. 6 mm×150 mm); the mobile phase acetonitrile-0.05%H3PO4 solution(13:87); detective wave-length 230 ran; column temperature 20℃. RESULTS The average recovery was 99.9 % , RSD =0.9%. CONCLUSIONS This method is available to quality control.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2002年第12期736-738,共3页
Chinese Journal of Hospital Pharmacy
