摘要
目的 研究新决明内脂与细胞内脂质代谢有关的生物学效应及分子机理。方法 首先用动物实验检查该化合物的生物学效应。再用细胞学方法研究该化合物对 3T3 L1前脂肪细胞分化的作用。最后用含有 10 0 0 0个人类基因和ESTs的高密度cDNA微阵列去研究新决明内脂如何改变HepG2细胞的基因表达图谱。结果 新决明内脂可减少大鼠的体重增加率 ,但对 3T3 L1前脂肪细胞分化的作用有限。HepG2肝细胞基因表达图谱结果显示新决明内脂调节了 4 6个与脂质代谢、蛋白代谢、细胞增生与凋亡等功能有关的基因。结论 新决明内脂的生理效果可能与一系列与脂质代谢有关的重要基因有关。
Aim To study the biological effect and molecular mechanism of neotoralactone on lipid metabolism in HepG2 cells. Methods Animal experiments were carried out to examine the biological effects of the compound. Differentiation assay was carried out to determine the effect of the compound on the 3T3-L1 preadipocyte. Finally, a high-density cDNA microarray containing about 10 000 human genes and ESTs were used to analyze the gene expression profiles of the HepG2 cells with and without neotoralactone treatment. Results Preliminary results showed that neotoralactone can decrease the increase of body weight of rats fed with high nutrient diet. As the differentiation assay showed that neotoralactone has minimal effect on the obese 3T3-L1 preadipocyte cell line, HepG2 heptocarcinoma cell line was used as the model system because one of the proposed mechanisms of weight reduction effect of Cassia obtusifolia is the alteration of lipid metabolism primarily taken place in the liver. The results showed that neotoralactone regulates a panel of genes related to lipid metabolism. Conclusion The effects of neotoralactone may be due to the regulation of a panel of important genes related to lipid metabolism.
出处
《药学学报》
CAS
CSCD
北大核心
2003年第1期62-66,共5页
Acta Pharmaceutica Sinica
基金
CityUniversityofHongKongthroughaninternalresearchgrant(CityUProjectNo .93 60 0 44 )
