摘要
目的 :检测面神经再生液的蛋白质成分及对运动神经元的生物营养活性。方法 :建立面神经再生室的动物模型 ,采集面神经再生液 ,用双向凝胶电泳技术对再生液进行蛋白组学的研究 ,并应用运动神经元培养的方法检验其生物活性。结果 :再生液中检测到 (85 0± 78)个蛋白质斑点 ,实验组培养神经元的胞体面积、突起长度及光密度值总体上大于对照组。结论 :再生液中蛋白含量丰富 ,种类繁多 ,面神经再生液对运动神经元确有营养活性作用。
Objective:To explore the protein components and the biological neutrional activity upon motor neuron,so that take a general?basic study on the nerve regeneration microenviroment which be important to facial nerve regeneration.Method:The animal model of rabbit facial regeneration microenviroment is estalished. Advanced technology of two dimentional polyacrylamide gel electrophoresis (2 D PAGE) was used to study on the structural proteins in the regeneration fluid ,as well as the motor neuron culture was used to test the biological activity in it.Result:850±78 protein spots were detected in the regeneration fluid.The body area?neurite length and OD value of expermental group are higher than that of control group.Conclusion:We got the protein image of facial nerve regeneration fluid by 2 D PAGE,and proved that many kinds and number of proteins in the regeneration fluid have neutrional activity on motor neuron.
出处
《临床耳鼻咽喉科杂志》
CAS
CSCD
北大核心
2003年第1期37-39,共3页
Journal of Clinical Otorhinolaryngology
基金
高等学校博士学科点专项科研基金资助项目(No.C96 0 6 30 )
关键词
面神经再生
运动神经元
蛋白质组
双向凝胶电泳
Facial nerve regeneration
Motor neuron
Proteome
Two dimentional gel electrophoresis
