摘要
在新疆克拉玛依小拐农场,从大沙鼠耳组织内查见的利什曼原虫,经NNN基培养11天,对原虫的前鞭毛体作超微结构观察。前鞭毛体多为柳叶状。其膜下微管数平均为80±9(68～111)个,微管直径为24nm,间距为15～28nm,质膜厚度为8nm,与吴传芬等和王捷等报道的甘肃沙鼠利什曼原虫的前鞭毛体有明显差别;在胞质内广泛分布着内质网,线粒体发达,高尔基体常见于线粒体一动基体复合体附近。在鞭毛基体上部有基板1和基板2。在胞质内还可看到脂滴和空泡。
Leishmania was obtained originally from ear tissues of big gerbil in Karamay, Xinjiang Uygur Antonomous Region. After culture of the parasites in NNN medium for 11 days, the specimens of leishmania promastigote were prepared for ultrastructural observation. The promastigote appeared in a villow leaf shape and the thickness of its plasma membrane was 8nm. The mean number of subpellicular microtubules sectioned in the nuclear level was 80 ± 9(68-111). The diameter of the micro tubule was 24nm, and the space between the microtubules was 15-28nm. Based on the above mentioned morphological characteristics, it is suggested that the promastigote of leishmania obtained from Karamay's big gerbil is quite different from the promastigote of L. gerbilli. Besides, the cytoplasma of the promastigote were rich in endoplasmic reticulum and mitochondria, while Golgi complex was usually seen nearby the mitochondria-kinetoplast complex. In the upper portion of flagellum basal body, basal plate 1 and basal plate 2 were detected.
出处
《地方病通报》
1992年第1期23-25,共3页
Endemic Diseases Bulletin
基金
国家自然科学基金
关键词
利什曼原虫
前鞭毛体
超微结构
Big gerbil
Leishmania
Promastigote
Subpellicular microtubule
Plasma membrane
Ultrastructure.
