摘要
目的 探讨犬颅脑火器伤后脑细胞内Ca2 + 变化的规律 .方法 建立犬脑枪弹伤致伤模型后 ,制备脑组织的活细胞悬液进行原代培养 ;应用特异性钙离子荧光指示剂Fluo 3 AM标记 ,在激光扫描共聚焦显微镜下检测伤后不同时期、不同部位脑细胞内钙离子的分布情况 .结果 培养得到了贴壁的犬颅脑火器伤后的脑组织活细胞 .并观察到 30min后细胞内Ca2 + 即开始升高 (P <0 .0 5 ) ,72h达到高峰 (P <0 .0 1) ,78h后又呈下降的趋势 (P <0 .0 5 ) ;而挫伤区和震荡区的差别不大(P >0 .0 5 ) .结论 颅脑火器伤后细胞内Ca2 + 的增加 ,在伤后的不同时期和不同部位的分布具有时空规律性 ,这可能是火器伤后脑水肿和一系列继发性脑损害的原因之一 .
AIM To study the regularity of intracellular Ca 2+ change after craniocerebral fire arm wounds in canines. METHODS After the canine craniocerebral missile wounds models were established, the living cells suspension liquids of the cerebral tissues were cultured. Intracellular Ca 2+ distribution was measured by calcium fluorescent probes Fluo 3/AM under laser confocal microscope at different periods and different positions of the canine brains . RESULTS The living cells of the cerebral tissues were cultured successfully after craniocerebral missile wounds. It was found that intracellular Ca 2+ increased obviously within 30 min( P <0.05), reached the summit at 72 h( P < 0.01 ),and then descended after 78 h( P <0.05).But the distinctions were unobvious between the regions of brain contusion and brain concussion .CONCLUSION The distribution of intracellular Ca 2+ demonstrated regularity at different periods,and at different positions of the brain.The changes of intracellular Ca 2+ was likely to be a pathophysiologic reason for the traumatic hydrocephalus and secondary lesion after craniocerebral fire arm wounds.
出处
《第四军医大学学报》
北大核心
2002年第23期2149-2151,共3页
Journal of the Fourth Military Medical University
基金
全军"九五"医学科研基金重点资助项目 (99 Z 1 4 6)
关键词
犬
颅脑火器伤
细胞内
实验观察
激光共聚焦显微镜
钙离子
craniocerebral fire arm wound
disease models ,animal
laser confocal microscope
calciumions
