摘要
目的 探讨缺血预适应对缺血脊髓的保护作用及其可能机制。方法 4 8只成年大耳白兔随机分为两组 ,每组 2 4只 ,建立脊髓缺血模型。缺血预适应组 (IPC组 )采用腹主动脉阻断 5min ,开放 15min的预适应方案后 ,再阻断 4 0min后开放。对照组常规阻断腹主动脉 4 0min后开放。分别于阻断前、阻断 4 0min后、开放后 2、8、2 4和 72h ,测定脑脊液超氧化物歧化酶 (SOD)、脊髓脂质过氧化物酶 (LPO)及脊髓组织含水量 ,并行双后肢神经功能评分。结果 IPC组各时相脑脊液SOD活性及神经功能评分显著优于对照组 (P <0 0 5 ) ,脊髓LPO含量及组织含水量明显低于对照组 (P <0 0 5 )。结论 缺血预适应通过调动与增强脊髓组织内源性抗损害机制对缺血脊髓发挥保护作用。
Objective: To evaluate the effects and possible mechanism of ischemic preconditioning (IPC) on spinal cord ischemic injury. Methods: The study consisted of two groups of mature rabbits. A spinal cord ischemia model was established. IPC group (n=24), received clamping abdominal aorta 5 minutes and then de-clamping 15 minutes and finally clamping again for 40 minutes. Control group (n=24), received 40 minutes clamping only. SOD in cerebrospinal fluid, LPO in spinal cord and content of water of spinal cord were measured at six different time intervals: before clamping after de-clamping 0, 2, 8, 24 and 72 hour. Nervous function of lower extremities was observed. Results: The activities of SOD in IPC group was higher (P<0.05), the state of nervous function was better in IPC group. The level of LPO and content of water of spinal cord in IPC group were lower. Conclusion: IPC can offer protection for spinal cord ischemia injury. The possible mechanism of IPC may be inhibit in of the oxygen free radicals, thus decreases the ischemia-reperfusion injury of spinal cord.
出处
《中华胸心血管外科杂志》
CSCD
北大核心
2002年第6期359-361,共3页
Chinese Journal of Thoracic and Cardiovascular Surgery
