摘要
目的:采用动态相关性分析法研究炒决明子中α-葡萄糖苷酶抑制作用的潜在活性成分。方法:利用大孔吸附树脂对不同化合物的吸附与解吸速率差异,采用不同浓度乙醇对炒决明子提取物进行梯度洗脱,结合UHPLC-Q-TOF-MS/MS分析不同浓度乙醇洗脱液的化学成分峰面积,建立炒决明子化学成分量[X]矩阵;通过不同浓度的乙醇洗脱液对α-葡萄糖苷酶的抑制作用,建立炒决明子化学成分效[Y]矩阵;采用PLS模型对[X]矩阵和[Y]矩阵进行相关性分析,预测炒决明子中抑制α-葡萄糖苷酶活性的有效成分,并对其中部分成分进行活性验证。结果:建立了可靠的PLS模型(正离子:R^(2)X=0.753,R^(2)Y=0.998、Q^(2)=0.982;负离子:R^(2)X=0.833,R^(2)Y=0.999、Q^(2)=0.993)。在正、负离子模式下分别预测到炒决明子中抑制α-葡萄糖苷酶活性的有效成分有86个和64个,最终鉴定出31个有效成分,主要为蒽醌类、黄酮类和萘并吡喃酮类成分。实验验证了槲皮素和山柰酚的活性,其IC50值分别为4.5μg/mL和7.6μg/mL。结论:采用大孔吸附树脂动态吸附解吸方法,解决了静态相关性分析的重线性问题,建立一种复杂体系中有效成分的发现方法,并证实炒决明子具有抑制α-葡萄糖苷酶活性。鉴定的有效成分主要为蒽醌类、黄酮类和萘并吡喃酮类成分。
Objective:This study aims to investigate the potential active components responsible forα-glucosidase inhibition in fried CASSIAE SEMEN using dynamic correlation analysis.Methods:Differences in the adsorption and desorption rates of different compounds on macroporous adsorption resin were utilized to perform gradient elution of fried CASSIAE SEMEN extract with ethanol at different concentrations.The chemical components amount matrix[X]was established by analyzing the peak areas of compounds in the eluates using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(UHPLC-Q-TOF-MS/MS).The inhibition ofα-glucosidase by eluates of different ethanol concentrations was measured to establish the bioactivity matrix[Y].Partial least squares(PLS)regression analysis was then applied to correlate matrix[X]and[Y],predict the effective components withα-glucosidase inhibitory activity in fried CASSIAE SEMEN,and verify the activity of selected compounds.Results:Reliable PLS models were established(positive ion mode:R^(2)X=0.753,R^(2)Y=0.998,Q^(2)=0.982,negative ion mode:R^(2)X=0.833,R^(2)Y=0.999,Q^(2)=0.993).A total of 86 and 64 active compounds were predicted in the positive and negative ion modes,respectively,with 31 active components ultimately identified.These components were mainly anthraquinones,flavonoids,and naphthopyranones.The activities of quercetin and kaempferol were verified with IC50 values of 4.5μg/mL and 7.6μg/mL,respectively.Conclusion:The dynamic adsorption-desorption method using macroporous adsorption resin effectively addressed the multicollinearity issues inherent in static correlation analysis and provided a novel approach for discovering active components in complex systems.The results confirm that fried CASSIAE SEMEN possessα-glucosidase inhibitory activity,primarily attributed to anthraquinones,flavonoids,and naphthopyranones.
作者
陈佳美
崔红静
梁杰豪
程佳丽
姜丽
张启云
李冰涛
曾治君
徐国良
CHEN Jiamei;CUI Hongjing;LIANG Jiehao;CHENG Jiali;JIANG Li;ZHANG Qiyun;LI Bingtao;ZENG Zhijun;XU Guoliang(Research Center for Differentiation and Development of Traditional Chinese Medicine Basic Theory,Jiangxi University of Chinese Medicine,Nanchang 330004;School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004;Jiangxi Provincial Key Laboratory of Chinese Medicine Etiopathogenesis,Nanchang 330004;Jiangxi Provincial Key Laboratory of Pharmacology of Chinese Medicine,Nanchang 330004)
出处
《中药药理与临床》
北大核心
2026年第3期96-106,共11页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金(编号:82060826)。
