摘要
The scallop male gonad(SMG)is the primary edible byproduct of the scallop P.yessoensis during scallop adductor muscle processing.SMG powders showed obvious antifatigue effects in a dose-dependent manner from 50 to 200 mg/kg/d,as reflected by a 40%-80%increase in exhausted swimming time and a 10%-90%increase in glucose,liver glycogen,and muscle glycogen content compared to those of the Con-S group.Moreover,the SMG powders could maintain the orderly arrangement of muscle tissue after acute vigorous exercise.Moreover,compared with those in the Con-S group,the SMG powders alleviated oxidative stress,as reflected by the downregulation of NAD+/NADH in muscle tissue and the increase in ATP content in muscle tissue by 0.2-fold and SOD content in serum by 0.1-fold.Additionally,protein and amino acid decomposition was alleviated,as reflected by a 14%-22%decrease in BUN as the amount of SMG powder increased.The 10%-12%decrease in the accumulation of BLA in the SMG-treated group corresponded well to the 11%-20%decrease in the LDH level in the serum.In addition,SMG powders downregulated the inflammatory cytokines IL-1β and IL-6 by 35%and 46%,respectively,compared with those in the Con-S group at a dose of 200 mg/kg/d.The antifatigue-related signaling pathways PI3K/AKT,NF-κB,and NRF2/HO-1 could be well regulated by SMG,which could be associated with high antioxidant-related amino acid contents(25.76%)and branched-chain amino acid contents(14.61%)in SMG,indicating that the development of food administration could increase exercise tolerance.
基金
the National Natural Science Foundation of China(U22A20538).
